《致病菌细胞的近红外光谱法检测限研究》-毕业论文（设计）.doc

摘 要 精品 PAGE II 致病菌细胞的近红外光谱法检测限研究 食品与生物工程学院 指导教师 摘　要 近红外光谱检测技术是近年来发展最快的检测技术之一，具有无损、快速、高效、方便和环保的特点。本文以对数生长期的沙门氏菌、单增李斯特菌等致病菌为研究对象，考察近红外光对致病菌细胞的作用，分析致病菌细胞的近红外光谱的图谱，利用基于主成分分析技术的投影判别分析对致病菌细胞的鉴别和检测限进行了研究，得到了预期结果。 1.无菌操作，采用液体培养基移种技术对沙门氏菌、单增李斯特菌进行分离培养，研究沙门氏菌、单增李斯特菌在营养琼脂培养基、含0.6%酵母膏的胰酪胨大豆琼脂（TSA—YE）培养基的生长情况。结果表明，沙门氏菌、单增李斯特菌均能生长良好。 2.采用光电比浊法，对沙门氏菌的对数生长期进行研究。通过比较沙门氏菌的生长曲线图，研究其生长趋势，确定了沙门氏菌的对数生长期，最后选定16h为沙门氏菌的培养时间。 3.根据不同浓度梯度的沙门氏菌、单增李斯特菌的全细胞、细胞壁和细胞质的近红外光谱，利用基于主成分分析技术的投影判别分析，研究两种菌的近红外光谱鉴别方法，以及检测限。结果表明，。 关 键 词：近红外光谱、沙门氏菌、单增李斯特菌、检测限 Research on the near-infrared spectrum detection limit of Pathogenic bacteria cells College of Food and Biological Engineering Rong zong-you Liu Jian-xue Professor ABSTRACT Near-Infrared spectroscopy detection technique is one of the fast developing technology，which is nondestructive, fast, efficient, converient, and environmentally friendly. The thesis explores the action mechanisms of near-infrared spectroscopy in the detection of pathogenic bacterias, analyses the near-infrared spectral of pathogenic bacteria cells, taking Salmonella and L.monocytogenes as the research object which is in the logarithmic growth phase, using projection discriminant analysis based on PCA to identification of the pathogen bacteria cells ,as well as the examination limit for a study to get the expected result. 1. Aseptic technique, Research the Growth situation of Salmonella and L.monocytogenes in both Nutrient agar culture medium and Including 0.6% yeast paste pancreas cream peptone soybean agar-agar culture medium(TSA—YE). The results show that，Salmonella and L.monocytogenes are not high to the nutrition demand and Can grow well in both Nutrient agar culture medium and Including 0.6% yeast paste pancreas cream peptone soybean agar-agar culture medium(TSA—YE). 2. Carried out a research into logarithmic growth phase of Salmonella and L.monocytogenes, taking the use of photoelectric turbidimetry. In this study, the growth curves of Salmonella and L.monocytog