《致病菌细胞的近红外光谱鉴别方法研究》-毕业论文（设计）.doc

摘 要 PAGE II 精品 致病菌细胞的近红外光谱鉴别方法研究 食品与生物工程学院 指导教师 摘　要 致病菌,即能够导致人类疾病或危害人类健康的细菌。致病菌污染食品可导致食物中毒与食源性感染，是食品安全的重大隐患。近红外光谱技术(NIRS)是一种高效快速的现代分析技术,是近年来发展最快的检测技术之一，具有无损、快速、高效、方便和环保的特点。 本文以沙门氏菌和单增李斯特菌为代表，采用平板划线法对沙门氏菌和单增李斯特菌的分离培养进行研究。结果表明，用亚硫酸铋琼脂（BS）培养基、改良的MC Bride琼脂（MMA）培养基可将沙门氏菌、单增李斯特菌从杂菌中很好的分离开来；用光电比浊法，对沙门氏菌的对数生长期进行研究。通过比较沙门氏菌的生长曲线图，研究其生长趋势，确定了沙门氏菌的对数生长期，最后选定16h为沙门氏菌的培养时间；以对数生长期的沙门氏菌、单增李斯特菌等致病菌为研究对象，考察近红外光对致病菌细胞的作用，根据不同浓度梯度的沙门氏菌、单增李斯特菌的全细胞、细胞壁和细胞质的近红外光谱图谱，利用基于主成分分析技术（PCA）的投影判别分析，研究两种菌的近红外光谱鉴别方法。结果表明，不论是利用全细胞、细胞壁还是细胞质的近红外光谱分析，都可以将大肠杆菌和单增李斯特菌区分开来。 关 键 词：近红外光谱，沙门氏菌，单增李斯特菌，主成分投影分析，鉴别方法  RESEARCH ON THE NEAR-INFRARED SPECTROSCOPY DISTINCTION METHOD OF PATHOGENIC BACTERIA CELLS Food and Bioengineering College Rong Zongyou Tutor Liu Jianxue ABSTRACT The pathogenic bacteria，which can cause the human illness to get sick or harm to human health of bacteria. Pathogenic bacteria contaminated food can cause food poisoning and food-borne infection，which is the significant hidden danger of food security. The near-infrared spectroscopy (NIRS)is one of the fastest growing testing technology，which take on the characteristic of nondestructive, fast, efficient, convenient and environmentally friendly. In this paper, we take the Salmonella and Listeria monocytogenes as the representative, using the methods of Plate crossed separation research the isolation and culture of Salmonella and L.monocytogenes. The results showed that，Salmonella and L.monocytogenes can be easily identified from Mixed bacteria With bismuth sulfite agar (BS) medium, modified MC Bride agar (MMA) medium. Taking the use of photoelectric turbidimetry, the logarithmic growth phase of Salmonella was researched. In this study, the growth curves of Salmonella were compared to their growth chart, from which we can see the growth trend of Salmonella. The logarithmic growth phase of Salmonella can be determinated. At last, 16h was selected to the Salmonella culture time. We take the logarithmic growth of Salmonella, Listeri