《无机离子浓度对以木糖为碳源的产氢发酵的影响》-毕业论文（设计）.doc

精品 PAGE PAGE 1 无机离子浓度对以木糖为碳源的产氢发酵的影响 摘要 生物制氢可利用生物质废弃物以实现二氧化碳的零排放，同时解决能源危机和环境污染。而利用废弃物制氢的研究大多集中在秸秆和有机废水上，关于废弃农作物的研究目前还没有见到报道。在我国每年产生千吨以上的废弃农作物，以木糖发酵制氢有很大的研究意义。另外，无机培养基中各个离子对发酵制氢有显著影响。然而由于各种离子都是以化合物的形态存在，在培养基中引入一种离子时并将带入另外一种离子，目前文献对各种离子浓度对发酵制氢的影响的讨论还不够透彻和详尽。 本文研究了营养物质NH4+,烟酸，VB1，还有HCO3-离子，金属离子对无机培养基厌氧发酵产氢的影响。结果表明，在反应温度为37℃，初始pH值为7.0的条件下，氮源NH4+的最佳浓度0.03-0.1158mol/L，终端产氢最大为338mL,烟酸浓度对产氢影响不大，VB1浓度越大越有利于产氢，并且有利于停滞时间的减少。HCO3-离子的最佳浓度为0.03mol/L,有利于停滞时间的减少。金属离子Fe2+和Ni2+对产氢影响不大，Mo2+的加入有利于产氢。反应结束后的pH值均比反应初始的低.。 关键词： 生物产氢 ，NH4+，烟酸，VB1 ， HCO3- ，Fe2+，Ni2+，Mo2+ IMPACT OF INORGANIC ION CONCENTRACTION ON THE FERMENTATIVE HYDROGEN PRODUCTION FROM XYLOSE AS CARBON SOURCE ABSTRACT Biohydrogen production with microorganisms can produce biohydrogen from waste of biomass without CO2 production, soluting the two problems of energy shortages, and environmental pollution. However，the study of biohydrogen production from waste almost was the sraw or the wastewater of organic, and have no reported about the xylose .It’s valuable to study the xylose for thousands tons of xylose were produced in our country. However, due to various ions are forms of existence, in compounds, introduced a kind of ions in the medium and will bring another kind of ion, at present literature on various ion concentration of hydrogen production of fermentation discussion of influence is not yet completed and detailed . In this paper, the effect of nitrogen source NH4+, nicotinic acid , VB1 . HCO3- and metal trace elements on fermentative hydrogen production by mixed cultures was investigated in batch test. The results showed that at 35℃ and initial pH 7.0, optimum concentration of NH4+ was 0.03-0.1158mol/L, the maximum hydrogen production was 338mL ; nicotinic acid concentration had little effect on hydrogen production ; the more concentration of VB1 , the better hydrogen production and the less dead time : optimum concentration of HCO3- was0.03mol/L and less dead time ;metal ions of Fe2+ and Ni2+ had little e