生物酶法合成L茶氨酸和聚谷氨酸的高效同步分离纯化.pdf

34 2 () Vol. 34 No. 2 2011 6 JOURNAL OF NANJING NORMAL UNIVERSITY ( Natural S ien e Edition) June, 2011 L , , , , (, 210046) [ ] ( glutam yl transpeptidase, GGT, EC 2322), . L ( poly glutam i a id, PGA ), . , L , , . GGT . [ ] L , , , [ ] Q789 [ ] A [ ] 100 146 16( 2011) Effective SimultaneousSeparation andPurification ofL theanine and PolyglutamicAcidFrom EnzymeCatalyzedSynthesis XinYinqiang, Lu Changyan, angQi, PuHefang, YinZhimin ( S hool of Life S ien es, Nanjing Norm alUn iversity, Nanjing 210046, Ch ina) Abstract: In enzym ati rea tion w hi h w as atalyzed by GGT, glutam ine and ethylam ine were used as substrates. The resu ltan t onsists of onsiderab le L thean ine and PGA, m ix ed w ith m ultip le mi purities in lude h lorid ion, ethylam ine, glu tam ine and glu tam ate. Through ationan ion tandem ex hange hrom atographyw ith respe tive types of resin, L thean ine w as effe tively separated for further purifi ation, a ompan ied w ith a quisition of valuable byprodu t PGA. Th is study provides the eviden e for large s ale produ tion ofL theanine w ith re om binant GGT. Keywords:L thean ine, PGA, GGT, ion ex hange L , , . , [ 1] . ( glutamyl transpeptidase, GGT, EC 2322) [ 2] , . , k- 12 ggt, pGEX [ 3] 4T1/ggt, BL21( DE3) , GGT . GGT , , . , L ( PGA ), . , D L , . , [ 4] , . GGT , L , , . : : , , , : . Em ai:l yinzh mi in@ njnu. edu. n 90 , : L 1 11 pGEX4T1/ggt.