氯化钠浓度对黄芩幼苗生长情况和生理指标影响Δ.PDFVIP

氯化钠浓度对黄芩幼苗生长情况及生理指标的影响Δ 1＊ 2 2 2# 郭菊梅 ，陈红刚 ，曹 瑞 ，杜 弢 （1. 陇西县种子管理站，甘肃陇西 748100；2.甘肃中医学院药学系，兰州 730000） 中图分类号 R927.2；S3 文献标志码 A 文章编号 1001-0408（2014）47-4497-03 DOI 10.6039/j.issn. 1001-0408.2014.47.27 摘 要 目的：探讨氯化钠（NaCl ）浓度对黄芩幼苗生长情况及生理指标的影响，为黄芩药材的大田生长提供依据。方法：采用盆 栽方式，待黄芩幼苗长至8～11cm 时，用1/2Hoagland 营养液兑加不同剂量的NaCl ，模拟盐胁迫环境，培养3d 后统计幼苗成活 率，并采用紫外-可见分光光度法测定叶绿素和类胡萝卜素的含量，用硫代巴比妥酸法测定丙二醛的含量，用电导法定膜透性。结 果：随着NaCl 浓度的增大，黄芩幼苗成活率、叶绿素和类胡萝卜素的含量均呈先升后降的趋势，丙二醛含量和相对电导率呈逐渐 升高的趋势；其中，NaCl 浓度为0.4％时，黄芩幼苗成活率、叶绿素和类胡萝卜素的含量均达到峰值。结论：黄芩幼苗的临界耐盐浓 度为0.4％，盐浓度＜0.4％时对黄芩幼苗的生长无明显影响，＞0.4％时盐害作用较严重。 关键词 黄芩幼苗；盐胁迫；成活率；膜透性；丙二醛；叶绿素；类胡萝卜素 Effect of NaCl Concentration on Seedling Growth and Physiological Indexes of Scutellaria baicalensis 1 2 2 2 GUO Ju-mei ，CHEN Hong-gang ，CAO Rui ，DU Tao （1.Seed Administration Station of Longxi County ，Gansu Longxi 748100，China ；2.Dept. of Pharmacy ，Gansu University of TCM ，Lanzhou 730000，China ） ABSTRACT OBJECTIVE ：To investigate the effect of NaCl concentration on seedling growth and physiological indexes of Scutel- laria baicalensis ，and to provide reference for the growth of S. baicalensis in large area. METHODS ：Seedlings of S. baicalensis potted ，8-11cm in height ，were cultured in 1/2 Hoagland nutrient solution with different concentrations of NaCl. Under simulated salt stress condition ，the survival rate of the seedlings was analyzed statistically after 3 days of culture. The contents of chlorophyll and carotenoid were determined by UV visible spectrophotometry ，and the content of malonaldehyde was determined by thiobarbitu- ric acid method. The membrane permeability was determined by conductivity method. RESULTS ：With the increase of NaCl concen- tration ，the survival rate of seedling became raised firstly ，and declined subsequently ，however ，the c