筛选、鉴定及降解能力的研究.PDF

中国农学通报 2011,27(7): 112-118 Chinese Agricultural Science Bulletin 高效纤维素降解菌短小芽孢杆菌（Bacilluspumilus）T-7的 筛选、鉴定及降解能力的研究 张立静，李术娜，朱宝成 （河北农业大学生命科学学院，河北保定071001） 摘 要：为了得到一株具有降解纤维素性能的产芽孢菌株，并获得菌株发挥最大降解特性所需的最佳培 养条件。采用加热富集芽孢菌及刚果红脱色圈的初筛方法，从菜地土壤、动物粪便、青贮饲料等样品中 分离筛选出41 株能够降解纤维素的产芽孢细菌。采用3 ，5-二硝基水杨酸比色定糖法(DNS)进行菌株纤 维素酶活测定，得到的菌株T-7 具有显著的降解能力，纤维素酶活力达1678.89 U/mL 。通过形态观察鉴 定、生理生化实验和16S rDNA 序列分析对其进行种属鉴定，鉴定T-7 菌株为短小芽孢杆菌（Bacillus pumilus）。研究了供试菌株T-7 的降解工艺，以10亿活菌/ 1 kg 的接种量接入玉米秸秆，并且添加辅助碳 氮源2%蔗糖+2%尿素时，在发酵8 天后对秸秆中纤维素的降解率达40.34%。研究结果为纤维素的生物 降解发掘了新的菌种资源，并为秸秆的大规模降解利用奠定了基础。 关键词：芽孢；纤维素；筛选；鉴定；降解能力 中图分类号：S182 文献标志码：A 论文编号：2010-2695 Screening,IdentificationandDegradationConditionsofCellulose DecomposingBacteriaBacilluspumilusT-7 Zhang Lijing, Li Shuna, Zhu Baocheng (CollegeofLifeScience,AgricultureUniversityofHebei, Baoding Hebei 071001) Abstract: In order to screen strain degraded the cellulose, and obtain the best cultivation condition which made full use of degrading. More than 41 strains were isolated from vegetable field, animal manure and silage feed by heating gathering spore bacteria strains and Congo red culture medium transparent hydrolysis circle, and tested the cellulose strains through the method of DNS. The T-7 strain has the highest capability, its enzyme activity was 1678.89 U/mL. The mycelium shape and the bacterial colony, physiological and biochemistry experiments and determination of the sequence of 16S rDNA of the fungi had been done. The strain was identified as Bacillus pumilus. It showed that the inoculum concentration of 1 billion viable cells/1 kg corn stalk, 2% sugar and 2% urea