小鼠肝动脉重建对长时间冷保存移植肝存活率影响剖析.pdfVIP

2010 5 1 3 O rgan Transplant, M ay 2010, Vol. 1, No. 3 135 王国栋 ! ( hepati arterial re onstru tion, HAR) C57BL /6 68, ( old preservation tmi e, CPT) 1 hCPT 4 hCPT 8 hCPT 16 hCPT 16 h+ HAR () 4∀ UW ( ) ( ) , HAR 5, , CPT 1 h4 h8 h 12 d 7 /710 / 109 /9CPT 16 h 1, 36 h , 10% ( 1/ 10), CPT 16 h+ HAR 90% ( 9 / 10) , , (P 001) CPT 1 h4 h, CPT 8 h , CRT 16 h, CPT 16 h+ HAR, , HAR ! ; ; ; ; E ffec t of h epatic ar ter ial reconstruc tion in m ice on the survival rate of liver grafts follow ing longterm co ld preservation inju ry WANG GuodongD ep artm ent of Transp lan tation Surgery, F irstAff iliatedH osp ital of Sun Yatsen University, Guangz ou 510080, C ina Corresp onding aut or: WAN G Guodong, E mai:l wgdgd@ 163com A bstract! O bject ive T o study the effe t of hepati arterial re onstru tion ( HAR) on the survival rate of liver grafts follow ing longterm old preservation injuryM e thods Syngenei m ale C57BL /6 m i e ( n = 68) w ere d ivided into 5 groups: CPT 1, 4, 8, 16 h and CPT 16 h+ HAR groupL iver perfusion was flushed into portal vein w ith 4∀ UW solution and the grafts w ere preserved in 4∀ UW solutionO rthotopi liver trans p lantation ( OLT) w as perform ed w ith a om b ination of suture ( suprahepati vena ava) and uff anastom osis te hnique ( infrahepati vena ava and portal vein ) The bile du t was re onne ted through the intralum inal stentThem ethods for HAR in m i e in lude endtoside anastom osis of abdom inal aorta w ith hepati artery from donor livers to abdom inal aorta in the re ip ient Re ipient survival was follow ed up and hepato ellu lar injury w as evaluated by histologyH epato yte repli ation w as exam ined by mi m unoh isto hem istryR esu lts Survival rate on postoperativ