腺病毒介导的RNA干扰降低VEGF转录抑制血管形成的研究.PDF

7 4 () Vo.l 7 No. 4 2007 12 JOURNAL OF NAN JING NORMAL UN IVERSI Y ( ENG INEERING AND ECHNOLOGY EDI ION ) Dec, 2007 腺病毒介导的RNA干扰降低VEGF转录 抑制血管形成的研究 许剑锋, 沈维干, 彭琬昕, 李朝军 ( , 210097) [] VEGF siRNAA dH 1-siRNA /VEGF, HUVEC, HUVEC. Real-tmi e R -PCR , AdH 1-siRNA /VEGF (HUVEC) VEGF mRNA . , VEGF mRNA 50% , AdH 1-siRNA / 4 VEGFHUVEC. 9 d, 3115 @ 10 /LL, 28 @ 4 4 10 /LL, AdH 1-siRNA /VEGF 2115 @ 10 /LL. AdH 1-siRNA /VEGFHUVECM atrigel , HPF 10175, 10125, AdH 1-siRNA /VEGF HPF 7. AdH 1-siRNA /VEGF. [] , , RNA [] Q291 [] A [] 1672-1292( 2007) 04-0084-04 Study on Inhibitation of the Tube Formation byRNA Introduced by Adenovirusby its InterferenceW ith andDecrease ofVEGF Transcription Xu J ianfeng, S henW e igan, Peng W anx in, L i Chaojun ( Schoo l ofL ife Science, Nanjing Norm alUniversity, Nanjing 210097, China) Abstract: his study uses the hom ologous recomb ination technique to construct a recomb inant adenovirusAdH 1-siR- NA /VEGF targeting VEGF infects hum an endothelial cells (HUVEC), observes the interference of the adenovirus w ith the proliferation ofHUVEC in vitro and the tube form ation. he experiem ent of real-tmi e reverse transcription- PCR show s that this adenovirus can especially decrease level of the VEGF mRNA ofHUVEC. Com paredw ith control group, themRNA expression ofVEGF decreased to 50% . AdH 1-siRNA /VEGF can efficiently interfere the prolifeyat- ion ofHUVEC. N ine days after infection of aden