昆虫学报论文模板.doc

PAGE PAGE 7 禾谷缢管蚜和麦长管蚜玻璃管药膜法敏感毒力基线的建立 鲁艳辉，杨 婷，高希武基金项目: 基金项目: 国家重大基础研究规划（“973”计划）项目（2006CB102003）; 国家“十一五”支撑计划（2006BAD08A03 作者简介: 鲁艳辉, 女, 1978年11月生, 河北唐山人, 博士研究生, 研究方向为昆虫毒理学及分子生物学, E-mail: HYPERLINK mailto:luyanhui1211@126.com luyanhui1211@126.com ? 通讯作者Corresponding author, E-mail: HYPERLINK mailto:gaoxiwu@263. gaoxiwu@263. 收稿日期Received: 2008-07-07 （中国农业大学昆虫学系，北京 100193） 摘要: 【目的】建立禾谷缢管蚜Rhopalosiphum padi (Linnaeus)和麦长管蚜Sitobion avenae (Fabricius)对常用杀虫剂的相对敏感基线。【方法】从田间采集麦蚜在实验室内饲养30代以上，利用玻璃管药膜法测定其对杀虫剂的敏感度，每条毒力基线为2次以上独立测定数据合并后计算结果。【结果】用玻璃管药膜法建立了包括新烟碱类、吡啶类、氨基甲酸酯类、有机磷类和拟除虫菊酯类等共22个药剂品种对禾谷缢管蚜和麦长管蚜3 h的敏感毒力基线。禾谷缢管蚜对新烟碱类药剂吡虫啉和啶虫脒的LC50值分别为0.02 ?g/cm2和0.007 ?g/cm2；对吡啶类药剂吡蚜酮的LC50值为0.124 ?g/cm2；对氨基甲酸酯类药剂丁硫克百威、硫双灭多威、灭多威、抗蚜威、西维因的LC50值为0.0026～0.70 ?g/cm2；对有机磷类药剂三唑磷、丙溴磷、氧乐果、乐果、马拉硫磷、辛硫磷、敌敌畏、毒死蜱的LC50值为0.005～0.065 ?g/cm2；对拟除虫菊酯类药剂三氟氯氰菊酯、高效氯氰菊酯、溴氰菊酯、联苯菊酯、氰戊菊酯、氯氰菊酯的LC50值为0.033～0.240 ?g/cm2。麦长管蚜对新烟碱类药剂吡虫啉和啶虫脒的LC50值分别为0.15 ?g/cm2和0.12 ?g/cm2；对吡啶类药剂吡蚜酮的LC50值为0.41 ?g/cm2；对氨基甲酸酯类药剂丁硫克百威、硫双灭多威、灭多威、抗蚜威、西维因的LC50值为0.005～0.76 ?g/cm2；对有机磷类药剂三唑磷、丙溴磷、氧乐果、乐果、马拉硫磷、辛硫磷、敌敌畏、毒死蜱的LC50值为0.018～0. 关键词: 禾谷缢管蚜; 麦长管蚜; 玻璃管药膜法; 敏感毒力基线; 杀虫剂; 抗药性监测 中图分类号: Q965.9 文献标识码: A 文章编号: 0454-6296(2009)01-00-00 Establishment of baseline susceptibility data to various insecticides for aphids Rhopalosiphum padi (Linnaeus) and Sitobion avenae (Fabricius) (Homoptera: Aphididae) by the method of residual film in glass tube LU Yan-Hui, YANG Ting, GAO Xi-Wu (Department of Entomology, China Agricultural University, Beijing 100193, China) Abstract: 【Aim】 The objective of this research was to establish susceptible toxicity baselines of both Rhopalosiphum padi and Sitobion avenae to 22 insecticides. 【Methods】 The method of the residual film in glass tube with 3 h exposure to insecticides was employed for determining toxicity of insecticide to both wheat species, which were originally collected from different provinces of China during 2005?2007 and reared on wheat seedlings in laboratory for more than 30 generations. 【R