柱前衍生化高效液相色谱法测定去氧葡萄糖的含量.pdf

Chin J M ar D r ug s, 2011 Oc tober , Vol. 30 N o. 5 2011 10 30 5 2- 1, 2 1 1 1 1 1* 洪碧红 , 黄文文 , 尤小云 , 谢全灵 , 刘俊朋 , 易瑞灶 ( 1. , 361005; 2. , 361005) : 建立柱前衍生化高效液相色谱法测定2- 去氧葡萄糖(2-DG) 含量的方法采用 1- 苯基-3- 甲 基-5-吡 酮( PM P) 为柱前衍生化试剂, 将 2-DG 在碱性条件下衍生化后直接进样测定分离柱为Hypersil -1 ODS2 色谱柱(4.6 mm 250 mm, 5 m) , 流动相为100 mmol 醋酸铵缓冲液( pH 5. 5)-乙腈(7822) , 流 - 1 -1 速1.0 m min , 波长249 nm 2-DG 在 19.68~ 393.6 g m 浓度范围内与峰面积具有良好的线 性关系( r = 0. 9997) ; 其定量限( S/ N = 10) 和最低检出限( S/ N = 3) 分别为 7. 8 和 3. 1 ng; 平均回收率为 101. 21% , RSD 0.63% 该方法简便实用检测灵敏度高测定结果准确, 适用于2-DG 的质量控制 :2- 去氧葡萄糖;柱前衍生化; 高效液相色谱法 :R917 : A : 1002-3461(2011) 05-0044-05 Determinationof 2-deoxyglucose byhigh performance liquid chromatographywithprecolumnderivatization 1, 2 1 1 1 HONG B-i hong , HUANG Wen-wen , YOU Xiao-yun , XIE Quan-ling , 1 1* IU Jun-peng , YI Rui-zao ( 1. T he T hir d I nstitute of Oceanogr ap hy , State Oceanic A dministr ation, X iamen 361005, China; 2. College of M aterials , X iamen Uni ersity , X iamen 361005, China) Abstract: Objective An effective precolumn derivatization HP C method was developed to determine 2- deoxyglucose ( 2-DG) . ethods The 2-DG after being derived with 1-pheny-l 3-methyl-5-pyrazolone (PMP) in alkaline medium was injected directly for determination. T he determi