碱性成纤维细胞生长因子对大鼠椎间盘细胞凋亡及基质代谢的影响外科学骨外科专业论文.docxVIP

优秀毕业论文 精品参考文献资料 兰州大学硕士学位论文Effects 兰州大学硕士学位论文 Effects of basic Fibroblast Growth Factor on Intervertebral Disc Cell Apoptosis and Matrix Metabofism in Rats Abstract obiective To observe the effect of basic fibroblast growth factors(bFGF) on apoptosis and matrix metabolism ofrat intervertebral disc cell cultured in vitro． Methods Rat intervertebml disc cells were cultured in vitro in monolayer．The well--grown intervertebral disc cells of second generation were induced by anti·-Fas antibody to apoptosis，treated、析th bFGFs of different concentrations(1 0，1 00，1 000I-tg／L)．By using flow cytometry(FCM)，the apoptosis of the cells stained、vitll Propidium Iodide(PI)was Assayed．The level of hydroxyproline and proteoglycan in culture medium were detected、析th chloramines T method and DMB chromometry method． Results O)Compared with control group，the apoptotic ratio of the intervertebral disc cells caused by different concentrations(1 0，100，l 000ttg／L)of bFGFs had a significant difference(87．62-q：1 1．06％，53．30-a：9．23％，16．75+4．21％： P0．01)．②The content ofhydroxyproline and proteoglycan in bFGFs culture medium was significantly increased as the increase of concentration of bFGFs【hydroxyproline (6．71+0．33)，(9．12-土0．41)，(11．58+0．12)p．g／L，P0．01；proteoglycan(23．21+2．87)， (32．45+5．23)，(37．18+3．22)lxg／L，P0．01]．The concentration ofbFGFs was positively correlated with hydroxyproline and proteoglycan．(ra=0．972，P0．0 1： rb=0．907 P0．01)． Conclusion bFGF call inhibit the apoptosis and promot collagen and proteoglycan synthesis of rat intervertebral disc cell． Key words basic fibroblast growth factor；intervertebral disc cell；apoptosis； hydroxyproline；proteoglycan Il 兰州大学硕_ 兰州大学硕_上学位论文 缩 略词 缩写 英文全称 中文名称 PCD Programmed cel l death 细胞程序性死亡 bFGF basic fibroblast growth factor 碱性成纤维细胞生长因子 TGF transforming growth factor 转化生长因子 PDGF platelet-derived growth factor 血小板衍生生长因子 VEGF vascular endothelial cell growth factor 血管内皮细胞生长因子 IGF．1 insulin like growth factor-1 胰岛素样生长因子．1 FCM flow cytometry 流式细胞仪 MTT