高压静电场对钝顶螺旋藻的生物效应生物物理学专业论文.docxVIP

内蒙古大学硕士学位论文B10LOGICAL 内蒙古大学硕士学位论文 B10LOGICAL EFFECTS OF HIGH VOIJ’AGE S1ArIC ELECTRIC FIELD 0N SPIRULn寸A PLATENSIS ABSTRACT In this paper,the spirulina platensis were treated with high voltage static electric field，the biomass indexes of the treated algae strains ES—x(x=l-9)were compared with the original strain IS to investigate the biological effect of high voltage static electric fieled on spirulina platensis．The rusults indicated that the content of malondialdehyde(MDA)decreased，the growth speed increased significantly except ES-5，the content of chlorophyll a，beta-carotene，phycocyanin and the SOD specific activity were increased by 37．69％，32．41％，19．26％and 7．06 respectively． The ultraviolet absorption spectrum，SDSelectrophoresis and stability of the phycocyanin were measured to study the ES-8 and IS．The results showed that the position of the maximum absorption peak Was the same，the value of maximum absorption peak of ES一8 was higher than IS．The electrophoresis pattern of ES-8 and ES Was in agreement，both contained two parallel bands，which were the two subunits of the alga blue protein with the size of 1 7000Da and 20000Da． Compared with IS，ES一8 has a better stability in phycocyanin solution when the NaCI content is lower than 4％． Orthogonal experiment Was adopted to optimize the extraction of 万方数据 内蒙古大学硕士学位论文chlorophyll 内蒙古大学硕士学位论文 chlorophyll a and the aqueous two—phase extraction of the algae blue protein in spirulina platensis．The optimization conditions were established．The resultS showed that the extraction rate of chlorophyll a reached 1．545％when the solid-liquid ratio Was 1：500(g／rrm)，ultrasonic power was 80％and continued for 20 minutes．The purity of the algae blue protein Was 3．1 1 and the recovery rate reached 93．07％when the concentration of PEG2000 and tassium sodium tartrate solution Was 1 7％(w／w) and 2 1％(w／w)respectively,the pH value was 6．5． KEYWORDS：spirulina platensis；high voltage static electric field；biological effect； ultraviolet abso