1种食用菌细菌隐性污染栽培种的菌种分离研究.PDFVIP

中国农学通报 2015,31(16):62-65 Chinese Agricultural Science Bulletin 1种食用菌细菌隐性污染栽培种的菌种分离研究 刘盛荣，张维瑞，阮俊峰，江胜滔 （宁德师范学院生物系，福建宁德352100） 摘 要：研究1种以木屑为主要配方的食用菌细菌隐性污染栽培种的菌种分离。稀释涂布平板法分析细 菌总数。栽培种捣碎后悬于无菌水，分别以短时(5 min)涡漩振荡、摇床振荡(180 r/min) 、低温静置(4℃) 3 种方式处理；灭菌纱布过滤收集、无菌水淋洗木屑接种至PDA 斜面，培养使其生长。以未经处理木屑为 接种物作为对照。结果显示栽培种细菌数为15500 cfu/g ；对照（30 支）以及采用短时涡漩振荡处理所接 30 支斜面全部细菌污染；摇床振荡1、2 、3、4 天纯化率分别为20% 、30% 、46.67%及63.3% ；低温静置1、2 、 3、4 天纯化率分别为3.3% 、10% 、46.67%和60% 。以木屑为主要配方的细菌隐性污染栽培种悬于无菌水 经1~4 天振荡或浸湿再分离的基内菌丝分离法可有效分离纯化菌种。 关键词：食用菌；栽培种；细菌隐性污染；基内菌丝分离；菌种分离 中图分类号：S646.9 文献标志码：A 论文编号：2014-2512 InvestigationontheIsolationofPleurotussp.fromaBacteriaContamination Sawdust-basedProductionSpawn LiuShengrong,ZhangWeirui,RuanJunfeng,JiangShengtao (DepartmentofBiology,NingdeNormalUniversity,NingdeFujian352100) Abstract:Theworkaimedtoisolatethe Pleurotus sp.fromabacteriacontaminationsawdust-basedspawn. Thenumberofbacteriawasdeterminedwiththeplatecountassay.FortheisolationofPleurotussp.,thespawn wasinitiallysuspendedinsterilewaterandthentreatedasfollows:(1)vigorouslymixedonavortexmixer withinfiveminutes;(2)keptcontinuouslyshakingat 180r/minfor 1-4days;(3)kepttobestaticat4℃for1-4 days.Next,thesuspendedspawnwascollectedbyfilteringusingsterilegauze,andwashedwithsterilewater. Finally,eachslantwasinoculatedwithasawdust,allowingforgrowthat25℃.Asthecontrol,thesawdustwas directlyinoculatedintoslantswithonepieceofsawdustperslant.Thebacteriainthespawndeterminedwere 15500cfu/g.Alltheslantswerefoundtobecontaminatedinthecontrol.Withthespawnvigorouslymixedfor5 minutesbeforetheisolation,nopurifiedslantswereobserved.Withshakingof 1,2,3and4days,thesuccess ratewas20%,30%,46.67%,and63.3%,respectively.Withkepttobestaticat4℃for 1,2,