玉米赤霉烯酮全抗原的合成及鉴定-食品安全质量检测学报.PDF

5 3 Vol. 5 No. 3 2014 3 Journal of Food Safety and Quality Mar. , 2014 1,2 2 2 2 2 2* 张 新 , 陆晶晶 , 王 丹 , 周 爽 , 赵云峰 , 吴永宁 (1., 430024; 2., , , 100021) 摘 要: 目的 (ZEN)方法 ZEN (BSA), , - MALDI-TOF , ZEN 结果 BSA 69693.5 Da66297.7 Da, BSA ZEN 10.68 ; OVA ZEN 5.32 ; ZEN 结论 ZEN ZEN 关键词: ; ; - Preparation and characterization of artificial antigen of zearalenone 1 2 2 2 2 2* ZHANG Xin , LU Jing-Jing , WANG Dan , ZHOU Shuang , ZHAO Yun-Feng , WU Yong-Ning (1.College of Food, Wuhan Polytechnic University, Wuhan 430024, China; 2.Key Laboratory of Food Safety Risk Assessment, Ministry of Health, National Center for Risk Assessment of Food safety, Beijing 100021, China) ABSTRACT: Objective To synthesize the complete antigen of zearalenone (ZEN). Method ZEN was coupled with cationic bovine serum albumin (cBSA) via a Mannich reaction. Molecular weights of the complete antigen and carrier protein were measured by MALDI-TOF, respectively. And the performance of complete antigen was further validated by chequerboard titration using a commercial ZEN immunoassay kit. Results The molecular weights of the conjugate and BSA were 69693.5 Da, 66297.7 Da, respectively. There were 10.68 ZEN mole- cules attached to each BSA molecule, and 5.32 ZEN molecules attached to each OVA molecule. A positive re- sult of the immunoreaction between the synthesized complete antigen and the