构树叶中黄酮类化合物的提取及测定.PDF

Univ. Chem. 2018, 33 (12), 67−70 67 •化学实验• doi: 10.3866/PKU.DXHX201804029 构树叶中黄酮类化合物的提取及测定 ——介绍一个大学综合化学实验 * 陈媛梅 ，丁来欣 北京林业大学理学院，北京 100083 摘要：介绍一个大学综合化学实验。该实验是一个科研转化的大学生综合化学实验，内容包括芦丁吸收曲线和标准曲线 的绘制、超声波辅助溶剂提取构树叶中的黄酮并用分光光度法测定总黄酮。该实验分析植物样品中的有效成分，并综合 运用多种定量分析基本操作，满足农林院校相关专业对化学实验的特殊需求，有利于训练学生的综合实验技能，提高学 生创新能力，可作为非化学专业低年级分析化学实验课的综合实验。 关键词：构树；黄酮；超声波提取；分光光度法；综合化学实验 中图分类号：G64；O6 Extraction and Determination of Flavonoids from Broussonetia Papyrifera (L.) Vent. Leaves: A Comprehensive Chemical Experiment for College Students CHEN Yuanmei * , DING Laixin College of Science, Beijing Forestry University, Beijing 100083, P. R. China. Abstract: A new comprehensive chemical experiment for college students is introduced. This experiment is from the achievements of scientific research. It includes obtaining absorption spectrum and calibration curve of Rutin, and ultrasonic-assisted ethanol extraction and spectrophotometric determination of flavonoids from Broussonetia papyrifera (L.) Vent. Leaves. The experiment involves several basic analytical chemistry procedures and the effective components in plant sample are analyzed. Through the experiment, the students’ experimental skills, comprehensive and innovative abilities are improved; furthermore, the specialty requirements are satisfied in agriculture or forestry universities. Thus, it is recommended to be included in the comprehensive analytical chemistry laboratory for students of non-chemistry majors. Key Words: Broussonetia papyrifera (L.) Vent.; Flavonoids; Utrasonic-assisted extraction; UV-Vis spectroscopy; Comprehensive chemical experiment 为了满足高等农、林院校相关专业对化学实验的教学需求，加强学生对农、林生产中实际样品 的分析过程的认识，激发学生的自主探索和创造能力，将教师个人的科研成果[1]和实验教学结合起 来，在分析化学实验中开设了与农林学科相关的综合实验项目。 超声波法[2]与常规的溶剂提取相比，具有作用时间短、条件温和、提取率高、提取物的结构不被 破坏等优点，已广泛用于天然植物的有效成