蜡质芽孢杆菌AR156防治水稻纹枯病机理初探-中国生物防治学报.PDFVIP

30(1)107-112 中国生物防治学报 Chinese Journal of Biological Control 2014 年 2 月 蜡质芽孢杆菌AR156防治水稻纹枯病机理初探 陈刘军，俞仪阳，王 超，柯红娇，刘红霞，郭坚华* （南京农业大学植物保护学院/农作物生物灾害综合治理教育部重点实验室/江苏省生物源农药工程中心，南京 210095 ） 摘要：蜡质芽孢杆菌AR156 是一种防治多种土传病害的生物制剂，对水稻纹枯病具有较好的防治效果。本 研究通过温室试验方面验证菌株 AR156 的防病效果和促生作用，从细胞水平和基因水平初步揭示了菌株 AR156 防治水稻纹枯病的机理。结果表明，菌株AR156 对水稻纹枯病的温室防效达73.06% ，同时促进水 稻生物量增加 14.45%。菌株AR156 处理提高了水稻植株SOD、PAL 、POD 和CAT 等防御酶活性，增强 了OsPR1b、OsPR10、OsNPR1 和ZB8 等防卫相关基因的表达。接种立枯丝核菌Rhizoctonia solani HNW-21 之前用菌株AR156 预处理的水稻植株，SOD 和PAL 分别提前4 、2 d 出现活性峰；防卫相关基因均提前表 达，且表达时间延长，从而提高水稻对纹枯病的抗性。 关 键 词：蜡质芽孢杆菌；水稻纹枯病；生物防治；防御酶；抗病基因 中图分类号：S476 文献标识码：A 文章编号：1005-9261 (2014)01-0107-06 Preliminary Research of Mechanisms underlying Bacillus cereus AR156-mediated Resistance to Rhizoctonia solani in Rice ∗ CHEN Liujun, YU Yiyang, WANG Chao, KE Hongjiao, LIU Hongxia, GUO Jianhua （College of Plant Protection, Nanjing Agricultural University/Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education/Engineering Center of Bioresource Pesticide in Jiangsu Province, Nanjing 210095, China ） Abstract: Bacillus cereus AR156, a biocontrol agent against a variety of soil-borne pathogens, could effectively control rice sheath blight in field. Here, the biocontrol efficacy and growth-promoting role of strain AR156 on rice was tested under greenhouse conditions, and the primary mechanism of strain AR156 in controlling rice sheath blight was also studied on both cellular and gene levels. The results revealed that AR156 could control Rhizoctonia solani with an efficiancy of 73.06%, and there was a 14.45% biomass increase in AR156-treated rice plants compared with those non-treated ones.