铜绿假单胞菌制剂抑制鼻咽癌细胞体外增殖-南方医科大学学报.PDF

·544 · 南方医科大学学报（J South Med Univ ） 2012;32(4) 基础研究 铜绿假单胞菌制剂抑制鼻咽癌细胞体外增殖 王 剑，吴德华，陈龙华 南方医科大学南方医院放疗科，广东 广州 510515 摘要：目的观察铜绿假单胞菌（PA-MSHA）注射液对人鼻咽癌细胞株5-8F 及6-10B 的生长抑制作用，初步探讨其作用机制。方 法MTT 法检测鼻咽癌细胞体外增殖情况，流式细胞术检测细胞周期，Westem blotting 检测细胞周期及凋亡相关蛋白。结果 MTT 结果表明，与未加药组相比，加药组5-8F 及6-10B 细胞的增殖能力明显降低（P0.05），呈剂量和时间依赖性。细胞周期检 测发现药物处理后的肿瘤细胞发生G /S 期阻滞（P0.05）。Westerm blotting 检测发现药物处理后的两组细胞周期相关蛋白 1 cyclinD1、CDK4、CDK6 及凋亡促进蛋白Bax 表达上调，凋亡抑制蛋白Bcl-2 表达下调。结论PA-MSHA 能够抑制鼻咽癌5-8F 及6-10B 细胞株增殖，其机制可能是通过影响细胞周期调节并促进细胞凋亡。 关键词：铜绿假单胞菌；鼻咽癌；增殖；凋亡 中图分类号：R453.9; R739.6 文献标志码：A 文章编号：1673-4254（2012）04-0544-04 doi: 10.3969/j.issn. 1673-4254.2012.04.023 /kcms/detail/44.1627.R1816.018.html Pseudomonas aeruginosa vaccine inhibits the proliferation of human nasopharyngeal cancer cells in vitro WANG Jian, WU Dehua, CHEN Longhua Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China Abstract: Objective To investigate the inhibitory effects of Pseudomonas eruginosa vaccine (PA-MSHA) on the proliferation of human nasopharyngeal cancer cells and explore the possible mechanism. Methods MTT assay was used to determine the cell growth of human nasopharyngeal cancer cell line 5-8F and 6-10B in vitro treated with the vaccine. The cell cycle distribution of the cells was detected by flow cytometry, and the expression levels of apoptosis and cycle-related proteins were evaluated by Western blotting. Results PA-MSHA treatment significantly suppressed the proliferation of 5-8F and 6-10B cells in a time- and concentration-dependent manner compared with the control group (P0.05). The cells with PA-MSHA treatment exhibited a decreased percentage of cells entering S phase and a corresponding increase in G1 phas