焦亚硫酸钠对大鼠背根节神经元膜离子通道的影响-环境科学专业论文.docxVIP

山西大学2006扁碳：L学位论文 山西大学2006扁碳：L学位论文 摘要 的影响强于TTx．S钠通道。最后我们观察了SMB对DRG神经元上L 型高电压激活的钙(，c。。)电流的作用。结果显示，SMB对丘。。电流也有显 著增大作用。使其增大的半数有效浓度为lp．M。并且19tM SMB推迟了 七。。电流的激活和失活过程。增大了如。L电流的反转电位。改变了L型钙 通道选择离子的敏感程度。SMB改变了DRG神经元膜上三种离子通道 的电生理学特性，可能会由此引起一些DRG神经元的损伤或凋亡，增加 机体的疼痛感，进而可能引发～些神经系统方面的疾病。 关键词： 焦亚硫酸钠；膜片钳；背根节神经元；钾电流；钠电流；钙电 流；大鼠 中图分类号：Q424，R992 LJJ西大学2006届顶：匕学位论文 LJJ西大学2006届顶：匕学位论文 摘要 Ef佗cts of sodium metabisulfite on jon channels in rat dorsal root ganglion neurons Calling(Environmental Science) Directed by Meng Ziqiang ABSTRACT Sodium metabisulfite(SMB)is most commonly used as a preservative in food products，like as biscuit，chocolate，jam，sausage，salami，in many alcoholic liquor like as beer,wine，champagne etc，and in drugs such as parenteral amino acid solutions．SMB has cytotoxic and genotoxic effects． SMB can induce bronchoconstriction in asthmatic patients．Recent studies have reported that SMB can induce the CAs and the SCEs in human lymphocytes．SMB also might have neurotoxicity．Dorsal root ganglion (DRG)neurons are primary sensory neurons and transmit sensory information from peripheral regmns to the central nervous system．DRG neuron plays a important role in regul ating systemic activity．So we studied the effects of SMB on ion channels using the whole cell configuration of patch—clamp technique in acutely isolated DRG neurons．The results are follows： SMB increased the amplitudes of transient outward potassium currents (TOCs)and delayed rectifier potassimn current(k)in a concentration-and voltage—dependent malmer．Ten btM SMB did not affect the activation process of YOCs and shifted the steady—state activation curve of爪in the hyperpolarizing direction．SMB inhibited their inactivation process ofTOCs SMB also increased the amplitudes of TTX—S and TTX—R sodium ctlrrents in 山西大学2006 山西大学2006 J亓|；硕士学位论文 摘要 a concentration-and voltage—dependent manner．Ten uM SMB have little effect on the activation process of TTX—S sodium channel，however shift in the steady—-state inactivation of TTX