姜黄素和有氧运动诱导的AMPK激活对中老年大鼠骨骼肌细胞自噬的影响初探-运动人体科学专业论文.docxVIP

研究结论：1)8周的恒定负荷有氧运动、姜黄素及两者联合干 研究结论：1)8周的恒定负荷有氧运动、姜黄素及两者联合干 预都能在一定程度上维持骨骼肌重量，改善骨骼肌组织的病变，其机 理可能是通过激活骨骼肌AMPK，提高中老年大鼠骨骼肌AGT7、 LC3．b、LAMP．2基因的表达来实现。 2)姜黄素和恒定负荷有氧运动可能是通过激活骨骼肌AMPK去 诱导自噬这一机制来维持骨骼肌质量及改善骨骼肌组织病变。 3)8周恒定负荷有氧运动比起姜黄素和姜黄素联合恒定负荷有 氧运动对于缓解骨骼肌质量流失及改善肌组织病变的作用更明显，可 能是由于恒定负荷有氧运动能使骨骼肌维持着一种更适应的自噬水 平。 关键词：有氧运动，骨骼肌衰减症，AMPK，姜黄素，自噬 m 万方数据 ABSTRACTObjective：By ABSTRACT Objective：By establishing the middle-aged and old rats 8 weeks aerobic exercise and curcumin intervention model，constant load test movement and curcumin rats soleus AMPK activity changes after intervention and autophagy related gene expression，and using HE staining to observe the histopathologic changes of skeletal muscle of rats，to explore movement combination of curcumin induced AMPK activity changes on the influence of the middle-aged and old rats skeletal muscle cell autophagy． Speculated that the movement of autophagy and the mechanism of the curcumin prevent Sarcopenia． Methods：40 healthy male with batch 2 1 months SD rats，SPF (Specific pathogen Free，SPF)level of animals，cage．Adaptive feed for three days，randomly divided into 4 groups，were randomly divided into blank control group(BC，n 2 1 0)，quiet curcumin group(QC，n=1 0)，a constant load exercise+curcumin groups(EC，n=1 0)，a constant load movement(NE，n 2 1 0)．Each component 2 cage feeding，each cage 5 only．Movement group rats using treadmill exercise，establish curcumin animal experiment model combined with aerobic exercise training intervention mode，the slope is 0，1 5 m／min speed X 60 min(10ad intensity is equivalent to 70％V02max)，curcumin dose of 1 00 mg／kg IV 万方数据 weight，continuous weight，continuous training for five days a week，a total of 8 weeks training．20 hours after the end of the training time remove the hind limbs of rats soleus，calculate the relative weight of the rats soleus(SI)，using Elisa method to detect the soleus AMPK activity change，by real—time fluorescent quantitative PCR detection AGT7 mRNA，LC3一b mRNA， LAMP-2 mRNA expression，and from