酵母双杂交文库构建与TMEFF2相互作用蛋白筛选-营养与食品卫生学专业论文.docxVIP

Abstractsignal Abstract signal transduction mediated by Ca+or tyrosine protein kinase(TPK)． Unexpectedly，a new splicing variant sequence of TMEFF2 was obtained when full length cDNA of TMEFF2 WaS cloned．It Was showed that the absent 60bp sequence just lies in the seventh by sequence analysis．It is to be confLrmed by further experience about how the splice variant works and its proportion in different tissues and the distribution frequency in the population． Keyword：TMEFF2 gene，helve development，yeast two-hybrid，splice varianthomologous recombination，SMART ⅥI 缩写词表缩写词表 缩写词表 缩写词表 缩写词 英 文 中 文 AD transcriptional activating domain 转录激活结构域 Ade adenine 腺苷酸 Amp 锄pjcillin 氨苄青霉素 APS ammonium persulfate 过硫酸铵 BMP bonem orphogcnic protein 骨形态发生蛋白 BD DNA binding domain DNA结合结构域 bp base pair(s) 碱基(对) BSA bovine semm albumin 牛血清白蛋白 cDNA complementary deoxyribonucleic acid 互补DNA CNS central nervous system 中枢神经系统 CREB cAMP response element binding protein cAMP应答元件结合蛋白 ddH20 double distilled water 双蒸水 DMSO dimethyl sulfoxide 二甲基亚砜 DNA deoxyribonucleic acid 脱氧核糖核苷酸 dm deoxynucleoside 脱氧核苷三磷酸 triphosphate DTT dithiothreitol 二硫苏糖醇 E．coli Escherichia coli 大肠埃希菌 EDlA ethylenediamine tetraacetic acid 乙二胺四乙酸 ERK extracellular signal-regulated kinase 胞外信号调节蛋白激酶 EST expressed sequence tag 表达序列标签 His Histidine 组氨酸 IPTG Isopropyl··B·—D--thiogalactopyranoside 异丙基硫代半乳酸糖苷 LB Luria—Bertani medium LB培养基 Ⅸ 缩写词表Leu 缩写词表 Leu Leucine 亮氨酸 mRNA messenger ribonucleic acid 信使核糖核酸 M．MLv Moloney murine leukemia virus 莫氏鼠白血病病毒 OD optical density 光密度值 Oligo(dD oligode oxythymidylic acid 寡聚脱氧胸苷酸 PlAGE polyacrylamide gel electrophoresis 聚丙烯酰胺凝胶电泳 PCR polymerase chain reaction 聚合酶链反应 PEG polyethylene glycol 聚乙二醇 P口ES piperazine--N,N-bis·· 哌嗪一N，N’一双(2一乙 (2·ethanesulfonic acid) 磺酸) PMSF Phenylmethylsulphonylfluoride 苯甲基磺酰氟 RNA ribonucleic acid 核糖核酸 RT．PCR reverse transcription polymerse 逆转录聚合酶链反应 chain reaction SD synthetic dropout SD培养基 SDS sodium dodecyl sulfate 十二烷基硫酸钠 TBE tris／boric acid(buffer) Tris／硼酸电泳缓冲液 T