降脂通络软胶囊对膜性肾病大鼠的肾保护作用及对肾组 织α-actinin-4、ZO-1表达的影响-中医内科专业论文.docxVIP

万方数据 万方数据 目 录 中文摘要 ················································································1 英文摘要 ················································································4 英文缩写 ················································································9 研究论文 降脂通络软胶囊对膜性肾病大鼠的肾保护作用及对肾组 织 α-actinin-4、ZO-1 表达的影响 前言 ·············································································· 10 材料与方法 ····································································· 11 结果 ·············································································· 20 附图 ·············································································· 23 附表 ·············································································· 33 讨论 ·············································································· 36 结论 ·············································································· 41 参考文献 ········································································ 42 综述一 姜黄素的研究进展 ························································ 45 综述二 活血化瘀中药治疗膜性肾病的研究进展及其对 α-actinin-4、 ZO-1 的影响 54 致谢 ···················································································· 62 个人简历 ·············································································· 63 中 中 文 摘 要 PAGE 10万方数据 PAGE 10 万方数据 降脂通络软胶囊对膜性肾病大鼠的肾保护作用及对肾组织 α-actinin-4、ZO-1 表达的影响 摘 要 目的：本研究运用阳离子化牛血清白蛋白（cationic bovine serum albumin，C-BSA）尾静脉注射的方法成功复制膜性肾病（membranous nephropathy，MN）大鼠模型，并给予降脂通络软胶囊、盐酸贝那普利进 行干预，通过测定大鼠 24 小时尿蛋白定量、血生化指标，光镜、免疫荧 光、电镜观测大鼠肾脏病理变化，免疫组化检测 α-actinin-4、ZO-1 蛋白 及 real-time PCR 法检测 α-actinin-4、ZO-1 mRNA 在肾脏中的表达，来探 讨降脂通络软胶囊对膜性肾病大鼠的肾保护作用及对肾组织 α-actinin-4、 ZO-1 表达的影响，从而为临床治疗膜性肾病提供理论依据。 方法：选取健康 SD 大鼠 60 只，普通饲料喂养一周后，测定 24 小时 尿蛋白定量均在正常范围内，随机分为正常组（ZG）10 只，造模组 50 只。造模组预免疫 1 周后，尾静脉注射阳离子化牛血清白蛋白，每周三次， 共四周。测定 24 小时尿蛋白定量，确定模型复制成功后，随机分为模型 组（MG）、西药组（BG）、降脂通络低剂量组（JLG）、降脂通络中剂量 组（JMG）、降脂通络高剂量组（JHG），每组各 10 只。西药组给予盐酸 贝那普利（10mg/kg/d）研磨溶于 3ml 生理盐水灌胃，降脂通络低、中、 高剂量组分别给