金属离子对被孢霉发酵产油脂的影响及培养条件优化-生物工程专业论文.docxVIP

华 华 中 科 技 大 学 硕 士 学 位 论 文 II II Abstract Polyunsaturated fatty acids (PUFAs) play a significant role in human life and health, because they have so many physiological functions. Most of them are extracted from plants or fish oil. Due to the constraint of season, climate and production, it can not fully meet the demand of market . PUFAs from microbial fermentation can become an effective alternative source. Through 18S rDNA and ITS sequences analysis , it indicates that the strain Haikou 5 is Mortierella sp.. Oil components analysis by GC-MS show that the oil is consist of polyunsaturated fatty acids (AA and gamma-linolenic acid), as well as a high content of hexadecanoic acid, octadecanoic acid, 9-octadecenoic acid, docosanoic acid , tetracosanoic acid and so on. By doing the single factor experiment of adding different concentrations of metal ions, Mg2+ and Cu2+ cant get good results. Adding Ca2 + at 5×10-5 mol/L, AA and GLA production were increased by 21.65% and 48.76%. Adding Fe2+ at concentration of 5× 10-6 mol/L, can get a precentage of AA at 61.365, the AA yield is 1.927 g/L, content of GLA can reach 13.553%, GLA yield is 0.426 g/L. Adding 5 × 10-5 mol/L Fe2 (SO4)3, although the biomass is slightly inhibited, it can get a 27.029% higher of AA production than the control. Adding Mn2+ concentration at 8×10-4 mol/L, accumulation of biomass decreased slightly, but oil production (4.430 g/L), AA production (2.708 g/L) and GLA production (0.533 g/L) can reach the maximum, we can reach the purpose of getting high-yield of GLA and AA. Adding Zn2+at 1× 10-3 mol/L, percentage of AA is 72.111, and production of AA is up to 2.071 g/L, it can reduce post-purification process and expenses, which will have good prospects for industrial production of AA. Adding ZnSO4 at different day show us that : the second day to add will meet the largest growth inhibition , the third day adding will meet the biggest inhibition of GLA, and the fourth day will meet the maximum in