金属纳米粒子有序膜与聚集体的制备及其在SERS光谱中的应用-物理化学专业论文.docxVIP

which had been modified with (3-aminopropyl) trimethoxysilaine(APTES) previously. Then the orderly films have been cleaned by a plasma device under different conditions. Furthermore pyridine and dithiodipyridine were selected as SERS probe molecules to evaluate the cleaning effect on SERS substrates in order to determine a better cleaning conditions. Select glutathione as the main research object, gold nanoparticles with uniform size and shape were synthesized by hydroxylamine seed-mediated two-step growing method, and silver nanoparticles were synthesized by reducing silver nitrate with sodium citrate. SERS spectrocopic features of glutathione reduced form molecules were characterized by using these Au and Ag nanoparticles ordered film as SERS substrates. It is significant that glutathione SERS detection limit is so low to the level 10-13 M when silver nanoparticles ordered films on ITO were used as SERS substrates. The preparation conditions of Na2S-Au NPs aggregates were experimentally optimized. The Na2S-Au NPs aggregates prepared under such conditions show good stability. As a comparison, we also prepared GSH-Au NPs aggregates，and selected both two aggregrates as the SERS substrates to characterize SERS spectra of several amino acid molecules. All these work mentioned as above will help us to prepare SERS substrates with excellent properties and these new SERS substrates can help us to characterize more biological molecules effectively with SERS technique. Moreover it facilities us to investigate adsorption mechanism of biologically important molecules on such SERS substrate surface. It will also provide the effective approach and useful information for the research of structure–property relationships of relevant biomolecules in future. Key words:Surface-enhanced Raman scattering (SERS) spectra;Metallic nanoparticles ordered films;Metallic nanoparticles aggregates;Glutathione;Amino acid 上海师范大学硕士学位论文目录 上海师范大学硕士学位论文 目录 PAGE PAGE VI 目 录 摘 要 I ABSTRACTIII 第一章 绪