幽门螺杆菌重组Bb.doc

双歧杆菌介导的福氏志贺菌Bb-ipaB 疫苗的免疫机制研究** 王国富 薛士鹏**基金?项目：云南省教育厅基金重点项目( **基金?项目：云南省教育厅基金重点项目(编号：09Z0078) 【作者简介】王国富(1974 )，男(汉族)，云南人，副教授，主要从事抗感染免疫研究*【通讯作者】吴利先，E-mail: HYPERLINK mailto:w_lixian@163.com w_lixian@163.com（云南省大理学院下关校区基础医学院微生物学教研室，大理，671000） 【摘要】 目的 探讨双歧杆菌（Bifidobacterium bifidum,Bb）介导的福氏志贺菌(Shigella flexneri)Bb-ipaB疫苗的免疫机制。方法 雌性BALB/c随机均分为3组：疫苗组（Bb-ipaB）、Bb组和PBS组。每周灌胃免疫1次，共4次。未次免疫1周后检测小鼠血清特异性IgG、IgE、IgA、小肠粘液sIgA，称重检测胸腺和脾指数，MTT法检测脾细胞增殖以及ELISA法检测脾细胞因子IFN-γ和IL-4水平。结果 疫苗组血清特异性IgG、IgE、IgA、小肠粘液sIgA，胸腺脾指数，脾细胞增殖能力以及脾细胞因子IFN-γ和IL-4水平均明显高于对照组。结论 Bb-ipaB疫苗免疫小鼠后,可刺激小鼠产生高水平的特异性抗体和细胞因子，能促进脾细胞增殖。是一种具有开发潜能和研究价值的新型福氏志贺菌疫苗。 【关键词】双歧杆菌；福氏志贺菌；疫苗；免疫机制 【中图分类号】R378 【文献标识码】A Study on immune mechanism of recombinant Bb-ipaB vaccine of Shigella flexneri WANG Guo-fu, XUE Shi-peng，WU Li-xian. （Department of Microbiology of basic medical school, Dali College of Yunnan，Dali 671000, China） 【Abstract】 Objective To explore the immune mechanism in BALB/c mice by immunization with Bifidobacterium bifidum mediated Bb-ipaB vaccine of Shigella flexneri. Methods The female BALB/c mice were randomly divided into 3 groups: the first group of the mice were fed with the Shigella flexneri vaccine, the second group of the mice were fed with Bb, the third group of the mice were fed by PBS, once a week and total four time. At last, the change of antibody(IgG, IgE and IgA) in the serum, sIgA in the mucosa and cytokines of splenocytes( IFN-γ、IL-4) of the mice were detected by ELISA assay and the capacity of splenic cell proliferation were detected by MTT. Results The level of antigen-specific antibody(IgG、IgE、IgA) and cytokines of splenocytes IFN-γ、IL-4，the capacity of splenic cell proliferation and the thymus and spleen index of Shigella flexneri vaccine group were higher than these of controls. Conclusion Shigella flexneri vaccine Bb-ipaB can produce the antigen-specific antibody(IgG、IgE、IFN-γ、IL-4、IgA) and cytokines of splenocytes IFN-γ、IL-4 at a higher level after oral immuniza