不同方程计算药物小分子与蛋白结合常数的比较研究.pdf

第35卷 第9期 发 光 学 报 Vol.35 No.9 2014年9月 CＨINESEJOURNAL OF LUMINESCENCE Sept. ,2014 文章编号:1000鄄7032(2014)07鄄1114鄄06 不同方程计算药物小分子与蛋白结合常数的比较研究 * 郭 莹,刘保生 ,李志云,张丽惠, 吕运开 (河北大学 化学与环境科学学院,河北 保定 071002) 摘要:在Tris鄄ＨCl缓冲溶液(pＨ=7.4)中,分别研究了莫西沙星、诺氟沙星、氧氟沙星、恩诺沙星、洛美沙星、 环丙沙星与牛血清白蛋白的相互作用,利用计算药物小分子与蛋白结合常数的常用方程:Scatchard修正方 程、Lineweaver鄄Burk双倒数方程、双对数方程、修正的Stern鄄Volmer方程、改进的双对数方程,分别计算了各沙 星药物与蛋白的结合常数和结合位点数。 对计算结果进行比较,结果表明对于药物与蛋白为 1∶1的结合反 应,除Scatchard修正方程外其他方程的计算结果十分接近,其中改进的双对数方程的计算结果更加合理 准确。 关 键 词:牛血清白蛋白;结合常数;结合位点数;比较研究 中图分类号:O658.9 文献标识码:A DOI:10.3788/ fgx1114 Comparative Study of Binding Constants Between Small Molecule Drugs and Protein Calculated by Different Equation * GUO Ying,LIU Bao鄄sheng ,LI Zhi鄄yun,ZＨANG Li鄄hui,LYU Yun鄄kai (College of Chemistry Environmental Science,Hebei University,Baoding 071002,China) *Corresponding Author,E-mail:lbs@ Abstract:In Tris鄄ＨCl buffer solution (pＨ =7.4),the interactions of moxifloxacin,norfloxacin, ofloxacin,enrofloxacin,lomefloxacin,and ciprofloxacin with bovine serum albumin were studied, respectively. Using modified Scatchard equation, Lineweaver鄄Burk double reciprocal equation, double logarithmic equation, modified Stern鄄Volmer equation, and improved double logarithmic equation,the binding constants and binding points of drugswith bovine serum albumin were calcu鄄 lated,respectively. For the binding reaction of drug鄄protein ratio of 1∶1,the calculation resultsare very close except modified Scatchard equation,and the improved double logarithmic equation isthe most accurate. Key words:bovine serum albumin;binding constant;co