应用聚合酶链式反应.pdf

6 12 Vol. 6 No. 12 2015 12 Journal of Food Safety and Quality Dec. , 2015 - 16S rDNA * 严琼英, 林 霖, 叶秀玲, 陈 晶, 黄建飞, 刘丛丛, 杨国武 , 兰全学 ( , 518131) 摘 要: 目的 方法 DNA, - (polymerase chain reaction-denaturing gradient gel electrophoresis , PCR-DGGE), 16S rDNA 结果 PCR-DGGE , , 结论 关键词: ; ; 16S rDNA; - Analysis of swollen jelly by polymerase chain reaction-denaturing gradient gel electrophoresis and 16S rDNA techniques YAN Qiong-Ying, LIN Lin, YE Xiu-Ling, CHEN Jing, HUANG Jian-Fei, LIU Cong-Cong, * YANG Guo-Wu , LAN Quan-Xue (Shenzhen Academy of Metrology and Quality Inspection, Shenzhen 518131, China) ABSTRACT: Objective To analyze the reason of swollen brand jelly. Method Total microbial community genome were extracted from swollen and normal jelly and analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Bacteria was isolated by traditional plate technique and the 16S rDNA of isolated bacteria was amplified and sequenced. Observation the production of gas by inoculating the isolated strain into normal jelly. Result Both the DGGE profile and plate technique result revealed that only one species of bacteria could be detected, fungus was not de