基于荧光信号放大策略检测生物大分子和汞离子-分析化学专业论文.docxVIP

聊城大学硕士学位论文 聊城大学硕士学位论文 PAGE PAGE iii 生物传感器的检出限，线性动态范围涵盖 5 个数量级。我们利用这一高灵敏度、高选 择性的检测方法检测了实际样品中汞离子的含量。 关键词：信号放大；核酸探针；生物大分子；荧光检测 ABSTRACT In recent years,with the development of Genome Project and Protein Project, in clinical diagnostics, food safety, environmental protection and other social areas, more sensitive, accurate, economic and simple detection methods for biological macromolec- ules, have been the focus of Life Analytical Chemistry. Signal amplification detection technologies is a cutting-edge analytical techniques which based on various enzymes and nanoparticles, have realized highly sensitive detections of biological macromolecules. In this thesis, we developed a variety of novel fluorescence signal amplifying detection technology platforms which based on nucleic acid enzymes, nanoparticles and fluorescent nucleic acid probes. The developed methods were demonstrated to have very high sensitivity for quantitative analysis of protein and DNA detection. The main researches are presented as following: A label-free fluorescence assay for thrombin based on aptamer exonuclease pro tection and exonuclease III-assisted recycling amplification In this chapter, we have developed a simple and sensitive homogeneous fluorescence protein assay by Exo III-assisted recycling amplification-responsive cascade ZnPPIX/G- Quadruplex supramolecular fluorescent labels coupled with aptamer exonuclease protection. First, this novel sensing system is simple in design and can be easily carried out by only simple mixing and incubation,thereby avoiding the complex handling procedures. Also, the assay eliminates the washing and separation steps by utilizing exonuclease, which saves time. Secondly, the aptamer probe without any modification and specific label is involved, which decreases the experimental cost and the design complexity, and retains the high target binding activity. Third, this assay provides high sensitivity and selectivity. It permits the detection of as low as 0.2 pM h