基于糖谱法比较不同茶多糖结构特征及酶法修饰抗氧化活性研究-生物化学与分子生物学专业论文.docxVIP

Shanghai Shanghai Normal University Master of Philosophy Abstract Abstract The research and application of sugar and spectrum method of 6 kinds of tea polymccharides from established a strong specificity and high selectivity characterization of polysaccharide chemistry feature map of the new method and provide a train of thought for the study of tea polysaccharides from quality control strategy；at the same time，after enzymatic modification，combined with in vitro antioxidant activity index，further screened with strong activity of polysaccharides hydrolysis products，SO as to provide experimental basis for tea polysaccharides from the development ofhealth food． The main work is as follows： 1．Based on the central composite design and response surface optimization of tea polysaccharide extraction and separation of impurities Selection has higher accuracy of nonlinear mathematical model fitting method， applying central composite design of CCD method，response surface RSM in combination witll first reported the preparation of tea flower preferred．On the basis of single factor experiment，the best method results are：material liquid ratio 1：1 4．5，time 2．08，temperature 79．1．The absolute value of the difference between the predicted and experimental values is less than 5％．The results show that the established mathematical model has good predictive and selecting tea tree flower polysaccharide extraction conditions good reproducibility． Using boiling water extraction and alcohol precipitation method was used to extract 6 kinds of crude polysaccharide(artificial high selenium polysaccharide， artificial low selenium sugar,artificial blank polysaccharide，natural selenium rich polysaccharide，Deng village green tea polysaccharide，tea flower polysaccharide)， total sugar content from large to small artificial blank polysaccharidetea flower polysaccharideDeng cunlv tea polysaccharideartificially low selenium polysaccharideartificial high selenium sugarn