英文文献 真菌和细菌共培养产生氯代二苯酮.pdfVIP

1444 J. Nat. Prod. 2001, 64, 1444 1446 Notes Pestalone, a New Antibiotic Produced by a Marine Fungus in Response to Bacterial Challenge Mercedes Cueto,† Paul R. Jensen,† Chris Kauffman,† William Fenical,*,† Emil Lobkovsky,‡ and Jon Clardy⊥,‡ Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California at San Diego, La Jolla, California 92093-0204, and Department of Chemistry and Chemical Biology, Cornell University, Ithaca, New York 14850 Received May 30, 2001 The isolation and structure determination of a new chlorinated benzophenone antibiotic, pestalone ( 1), is described. The new compound was produced by a cultured marine fungus only when a unicellular marine bacterium, strain CNJ-328, was co-cultured in the fungal fermentation. The fungus, isolated from the surface of the brown alga Rosenvingea sp. collected in the Bahamas Islands, was identified as an undescribed member of the genus Pestalotia. The structure of 1, initially assigned with only modest confidence by combined spectral and chemical data, was confirmed by single-crystal X-ray analysis. Pestalone ( 1) exhibits moderate in vitro cytotoxicity in the National Cancer Institute’s 60 human tumor cell line screen (mean GI50 6.0 M). More importantly, pestalone shows potent antibiotic activity against methicillin-resistant Staphylococcus aureus (MIC 37 ng/mL) and vancomycin-resistant Enterococcus faecium (MIC 78 ng/mL), indicating that pestalone should be evaluated in advanced models of infectious disease. The development of resistance toward current antibiotics continues to be a significant problem in the treatment of infectious disease,14 and therefore the discovery an