基于微流控芯片的细胞电融合诱导小鼠体细胞重编程的去甲基化机制研究-眼科学专业论文.docxVIP

第三军医大学硕士学位论文 第三军医大学硕士学位论文 PAGE PAGE 1 缩略语表 英文缩写 英文名称 中文名称或注释 5mC 5-methylcytosine 5 甲基胞嘧啶 5hmC 5-hydroxymethylcytosine 5 羟甲基胞嘧啶 5hmU 5-hydromethyluracil 5 羟甲基尿嘧啶 AID Activation-induced cytidine deaminase 活化诱导胞苷脱氨酶 BSA Albumin Bovine V from bovine serum 牛血清白蛋白 BSP Bisulfite Sequencing PCR 亚硫酸氢盐测序法 CKAP2 cytoskeleton associated protein 2 细胞骨架相关蛋白 2 DAPI 4,6-diamidino-2-phenylindole 4,6-二脒基-2-苯基吲哚 DEPC Diethypyrocarbonate 焦碳酸二乙酯 DMEM Dulbecco’s modified eagle medium 改良的基础培养基 DMSO Dimethyl sulphoxide 二甲亚砜 ESC Embryonic Stem Cell 胚胎干细胞 EDTA Ethylenediaminetetradcetic acid 乙二胺四乙酸 FACS fluorescence-activated cell sorting 荧光激活细胞分选术 FSC Fused stem cell 融合多能干细胞 FBS Fetal bovine serum 胎牛血清 GFP Green fluorescence protein 绿色荧光蛋白 GFAP Glial fibrillary acidic protein 神经胶质纤维酸性蛋白 glucMS-qPCR Glucosylation of genomic 5hmC followed by methylation sensitive qPCR 葡萄糖基转移酶修饰后甲基 化敏感性 qPCR iPS induced pluripotent stem cells 诱导多能干细胞 LIF Leukemia inhibitory factor 白血病抑制因子 MBD4 methyl-CpG-binding domain protein 4 甲基化 CpG 结合域蛋白质 4 MEF mouse embryo fibroblast 小鼠胚胎成纤维细胞 PBS Phosphate buffered saline 磷酸盐缓冲液 PCR Polymerase Chain Reaction 聚合酶链式反应 PEG Polyethylene Glycol 聚乙二醇 PFA 4%paraformaldehyed 4%多聚甲醛 PM Pluse medium 电脉冲介质缓冲液 qPCR Realtime-time quantitative PCR 实时荧光定量 PCR RFP Red fluorescence protein 红色荧光蛋白 SCNT Somatic Cell Nuclear Transfer 体细胞核移植技术 TDG Thymine-DNA glycosylase 胸腺嘧啶 DNA 糖苷酶 Tet ten-eleven translocation 10-11 易位酶 DNA demethylation of somatic cells during the electro-fusion induced reprogramming using a microfluidic chip Abstract Background: Differentiated somatic cells can be reprogrammed into pluripotent stem cell through somatic cell nuclear transfer(SCNF), co-culture with stem cell extract, transduction of defined transcription factors and cell fusion, which has a great prospect in the field of regenerative medicine. However, SCNF is very difficult and its efficiency is very low, while co-culture with stem cell extract and transduction of defined transcription factors both