含药血清对体外培养早期人膝骨关节炎成纤维样滑膜细胞COX-2mRNA表达的影响-中西医结合临床专业论文.docxVIP

PAGE PAGE 4 结果：（1）所有的早期膝骨关节炎患者成纤维样滑膜细胞经不同含 药血清干预后均能检测到 COX-2mRNA。（2）采用 2-ΔΔCt 进行相对表 达量分析，生理盐水组的 COX-2mRNA 表达水平最高,与塞来昔布组、化 瘀祛湿组及补肾活血组比较有明显差异(P＜0.05)。（3）含药血清组间比 较:化瘀祛湿组与塞来昔布组两组间的 COX-2mRNA 表达量比较差异无统 计学意义(P＞0.05)，而以上两组与补肾活血组比较差异均有统计学意义 (P＜0.05)。 结 论 ：（1）所有的早期（Ⅰ-Ⅱ级）膝骨关节炎患者成纤维样滑膜 细胞经不同含药血清干预后均能降低 COX-2mRNA 的表达水平。（2） COX-2mRNA 作为早期骨关节炎炎症途径的发生、发展过程中一个可能 潜在的启动因子，而早期使用中药复方化瘀祛湿方对早期膝 OA 患者成 纤维样滑膜细胞中 COX-2mRNA 的表达具有一定的抑制作用，有望作为 防治 KOA 的有效方法，值得进行更深一步的研究。 关键词：膝骨关节炎,成纤维样滑膜细胞,COX-2mRNA,含药血清,体外 培养 The drug serum that contained Huayu Qushi Dec- oction on the effects COX-2 mRNA expression in early human knee osteoarthritis synovial fibrobla- st-like cells in vitro ABSTRACT Objective: By researching the drug serum that contained Huayu Qushi Decoction on the effects COX-2 mRNA expression in early human knee osteoarthritis synovial fibroblast-like cells in vitro, to explain the mechanism that Chinese medicine compound formula Huayu Qushi Decoction deal with the early human knee OA, and to explore the targets of Huayu Qushi Decoction therapyed OA, in order to Provide a reliable theoretical basis for the experiment to traditional Chinese medicine treatment of KOA. Methods: 48 healthy adult New Zealand white rabbits that each half of the male and female were choosed to number code. According to a random number table, there were randomly selected 24 were divided into four groups, each group had 6rabbits:group A(NS group),group B(Bushen Huoxue decoction group),group C(Huayu Qushi Decoction group), group D(celecoxib group).Before surgery cut cruciate ligament and medial collateral ligament degeneration rabbit model of knee OA. Four weeks after the start of administration modeling success, according to human and animal body surface area than the calculated equivalent dose animals to determine the daily dose gavage for 7 days. Normal saline irrigation with normal NS group . Standard rearing environment natural feeding . In the last day dose administered 90min after anesthesia by rab