反相高效液相色谱法测定维生素K2中七烯甲萘醌的含量.PDF

6 8 Vol. 6 No. 8 2015 8 Journal of Food Safety and Quality Aug. , 2015 K2 * 王德伟 , 游景水, 叶礼红 (, 519040) 摘 要: 目的 K2 (MK-7)方法 Diamonsil C18 (4.6 mm×250 mm, 5 μm), 50%/(0.1%)(95:5= V:V), 50% 1.0 mL/min, 270 nm, 40 ℃结果 MK-7 0.355~2.132 mg/mL , 0.9999 0.41%, 98.15%结论 , K2 MK-7 关键词: K2 ; ; Determination of menaquinone-7 in vitamin K by reversed-phase high 2 performance liquid chromatography * WANG De-Wei , YOU Jing-Shui, YE Li-Hong (By-Health Co. , Ltd., Zhuhai 519040, China) ABSTRACT: Objective To establish a method for the determination of menaquinone-7 (MK-7) products in the vitamin K2 by reversed-phase high performance liquid chromatography (RP-HPLC). Methods The samples were performed on C diamonsil (4.6 mm×250 mm, 5 μm) column with 50% methanol/water (containing 0.1% acetic acid) 18 (95:5=V:V) and 50% propane as mobile phase, the flow rate at 1.0 mL/min, the detection wavelength at 270 nm, and the column temperature at 40 ℃ . Results MK-7 had a good linearity in the range of 0.355~2.132 mg/mL, and the correlation coefficient was over 0.9999. The relative standard deviation of the repeatability experiment was 0.41%, and the recovery of the standard was 98.15%. Conclusion The method has a good accuracy, good reproducibility and simple operation, and can be appl