果糖自氧化体系在抗氧化活性组分筛选和评价中的应用研究-化学专业论文.docxVIP

重庆大学 重庆大学硕士学位论文 英文摘要 PAGE PAGE VI ABSTRACT The development and progression of diseases related to free radicals have been got lots of attention from researchers with the development of free radicals medicine and biology. Accordingly, the prevention and treatment of antioxidants on those diseases has become a hot topic. There are several deficiencies in the evaluation of antioxidants activity by traditional methods, which didn，t evaluate the comprehensive antioxiants activity because there is only a single type of free radical. In addition, researches on the antioxiants are devoted to the prevention and treatment of diseases related to free radicals and the generation of reaction oxygen species (ROS) is a complex and dynamic process in organism, currently methods established evaluating antioxidants activity are single and static system, so it is very siginificant to develop a dynamic and complex method which is similar to the generation and transition of ROS in organism. It is suggested that fructose has a faster autoxidation rate and the ability to trigger protein glycosylation compared with other sugars, such as glucose, gatactose and so on. There are a variety of active oxygen species (O2-·、HO2-·、·OH) generated from the process of fructose autoxidation under the catalysis of metal ions, and those ROS change into ·OH after a series of changes. In this thesis, a novel method of screening and evaluating antioxidants activity based on the ROS from the process of fructose autoxidation has been established because the generation and transition of ROS in fructose autoxidation is similar to the orgamism. Based on the principle that ·OH produced in the fructose autoxidation is able to oxidize benzoic acid to hydroxybenzoic acid, which has a strong fluorescence intensity, and the ROS could be eliminated by the antioxidants, which can decrease the fluorescence intensity quantitatively, the screening ration and antioxidants acivity were obtained. This method was applied to