龟鹿二仙汤含药药血清对体外培养SD大鼠成骨细胞增殖、分化与OPGODF系统的影响-中医学专业论文.docxVIP

万方数据 万方数据 中文摘要 龟鹿二仙汤含药药血清对体外培养 SD大鼠成骨细胞增殖、分化与OPG/ODF系统的影响 目的：探讨龟鹿二仙汤含药血清对成骨细胞(osteoblast，OB)增殖分化及骨保护 素(Osteoprotegerin ,OPG)、破骨细胞分化因子（Osteoclast differentiation factor, ODF）的影响。 方法：颅骨酶解法培养OB，分别予以10％ ～20％系列浓度的对照鼠血清或中药 血清培养，应用MTT法、对硝基苯磷酸盐法及茜素红染色方法观察体外培养成骨 细胞、碱性磷酸酶及矿化结节的变化,实时荧光定量PCR测定成骨细胞OPG、 ODFmRAN基因表达。 结果：含药血清组明显增加了成骨细胞数量、碱性磷酸酶的表达水平及矿化结节 的形成,作用随剂量的增加而增加；含药血清组可提高成骨细胞OPG mRNA的相对 表达量而对其ODF mRNA有抑制作用，与对照组相比有统计学意义。 结论：龟鹿二仙汤药物血清可以呈剂量依赖性地促进成骨细胞增殖分化；龟鹿二 仙汤药物血清在体外可通过上调成骨细胞OPG表达、抑制ODF表达来减少破骨细 胞的生成，抑制破骨细胞的活化，从而降低破骨细胞的骨吸收功能。 主题词：骨质疏松，成骨细胞，骨保护素，破骨细胞分化因子 I Abstract Effects of containing drugs blood serum of GuiLuErXianTang on the Proliferation and Differentiation and mRNA expressing of OPG and ODF of osteoblast in SD rats in vitro Objective ： To investigate the efects of the GuiLuErXianTang-treated pharmacological serum on the Proliferation and Differentiation and mRNA expressing of OPG and ODF of osteoblast in SD rats Methods：The OBs were isolated by the enzyme-digested assay then cultured．The OBs were treated in vitro with the pharmacological seram in a series of concentration，then used the method of MTT , para- nit ro-pheneye phosphate and the tinctorial method of alizarin Bordeaux to observe the effect of GuiLuErXianTang on such aspect s as the proliferation , ALP expression , and mineralization tuberculation of the Osteoblast .,The mRNA expression of OPG and ODF in osteoblasts was analyzed by real-time PCR. Results： GuiLuErXianTang medicine serum could obviously increace the quantity o f o s t e o b l a s t ， t h e l e v e l o f t h e A LP e x p r e s s i o n a n d m i n er a l i z at i o n tuberculation which is obviously improved by the close of GuiLuErXianTang. GuiLuErXianTang medicine serum could increase osteoblast OPGmRNA the relative expression of its ODF mRNA, compared with the control group were significantly different. Conclusion：GuiLuErXianTang medicine serum can present the ground of dose dependent to promote the osteoblast’s prolifer