观赏用柳树无性系再生体系的建立及其遗传转化的研究-植物学专业论文.docxVIP

山东师范大学硕士学位论文Establishment 山东师范大学硕士学位论文 Establishment of Plant Regeneration System and Transformation System of Ornamental Willow Clone Abstract SaZixX aureo-pendula e1．“J1010”belongs to Salicaceae Sa般．it is one catO氇efy of hardwood，its leaves are like the shape of needles．Its tress is drooping， yellow in color,elegancy and bright(brancMet is kelly in color in growing season and golden in dormacy season 1．The disease-resistance is strong．11坞male plant has no wadding in spring so that it brings no environmental pollution．S．XduMo-pe确uk c1．JlOlOalso grows fast，It iS fond of light，humid climate and fertile soil，but it has stong adaptability to soft，is able to endure the damping，and especially争ows well neat也e river．What，s mote，it can also grow normally in fertile soil and dry hypsography wi出the best strength of the budding． TKs thesis studies the tissue culture and regeneration of 爱xaureo-pendula c1．JlOlffand investigates the cell morphology On the calli of the different doses and hormones by paraffin section，aiming to study different effectcal conditions of the regeneration of theXaumo-penduc1．‘JlOlO．Through the study of the genetic transformati∞of the置Xaureo-pendulacl．“J10lO”with Agrobacterium turaefac缸ns． after screening and culturing by the antibiotic，this experiment obtains transgenetic resistant calli．Morever,it conducts primary test to the mmsgenetic calli by the histoehemistry dyeing of GUS． 1．Establishment of the tissue culture and regeneration system Altenative usage of MS+6·BA0．1mg／L+NAA 0．2mg／L and MS+6·BA 0．5mg／L +NAA0．1 m#L iS the most suitable way for the propagation．Taking the leaves and stem as the explants。MS+BAl．0mg／L+NAA0．1 mg／L and 6-BAI．0rag／L+2，4-D0．1 mg／L are preferable for the induction calli．1／2MS+NAA0．3 maL is the most suitable medium for the rooting． 2．Establishment of the embryo calli The result shows that adding the 1．0～2．0mg／L6一BA and O．1～O．5mg／L NAA to the medium in the proess of inducing the calli may induce embryo calli． 3．Establishme