固定化微生物反应器处理含镍废水的研究.docVIP

PAGE \* Arabic 2 收稿日期： 基金项目：陕西省科技厅基金资助项目(2008K08-03)；陕西省教育厅基金资助项目(09JK656) 作者简介：庞秀芬，（1967-）女，讲师，研究方向为环境工程。Email：pxfen@126.com. 联系电话微生物反应器处理含镍废水的研究 庞秀芬1 段若依2 梁民博1 田萍1 (1.西安理工大学应用化学系，西安 710054 2.天津大学环境学院，天津 300350） 摘 要 从受污环境中分离筛选出对Ni2+有去除活性的产朊假丝酵母菌y188。采用响应曲面法对游离细胞去除废水中Ni2+的条件进行优化。在35℃，pH 7.0，130 r/min振荡培养25 min的条件下，y188细胞对模拟废水中Ni2+的去除率达到98.49%。以y188为菌种、废弃煤渣颗粒为载体组建固定化细胞生物反应器。该反应器在15～45℃、pH 2～8的条件下均保持良好的除Ni2+活性，对Ni2+浓度为10 mg/L的模拟废水可连续处理8批，Ni2+的去除率保持在91% 以上，对Ni2+浓度为13.74 mg/L的实际废水连续处理6批，Ni2+的去除率保持在93% 以上。出水Ni2+浓度均1.0 mg/L，达到国家电镀污染物排放标准。显微观察结合解吸实验表明，y188细胞在30 min内对废水中Ni2+的去除主要是吸附除Ni2+。 关键词 产朊假丝酵母菌；废弃煤渣；固定化细胞；微生物反应器 中图分类号 X52 文献标识码 A 文章编号 Study on Removing Nickel fromWastewater by Immobilized Microbial Reactor Pang Xiufen 1, Duan Ruoyi2, Liang Minbo1, Tian Ping1 Department of Applied Chemistry, Xian University of Technology , Xian 710054, china; 2. Department of Environment, Tianjin University, Tianjin 300350 , china ) Abstract Candida utilis y188 which had removal activity was separated from polluted environment .Response Surface methodology (RSM) was used to optimize the removal conditions of Ni2+. they were pH 7.0, temperature 35℃, reaction time 25 min, rotate speed 130 r/min. The removal rate of Ni2+ by y188 was 98.49%. Using y188 as strains, The microbial immobilized reactor was created for abandoned coal cinder as carrier. Under the condition of temperature 15～45℃ and pH 2～8,The reactor had good removal activity of Ni2+.The microbial reactor treated simulated wastewater containing 10 mg/L Ni2+ 8 groups. The removal rate of Ni2+ was above 91%. It treated actual wastewater containing 13.74 mg/L Ni2+ 6 groups . The removal rate of Ni2+ was remained above 93%. The concentration of Ni2+ was lower than 1.0 mg/L, and achieved the national electroplating pollutants emission standards. Microscopic observation and desorption experiment showed that removal of Ni2+ from wastewa