正交试验优化复凝聚法制备液体香精微胶囊工艺-食品科学.PDF

34 2015, Vol.36, No.10 食品科学 ※工艺技术 正交试验优化复凝聚法制备液体香精微胶囊工艺 1 1 2 1 1 包清彬 ，王春丽 ，孔 凌 ，何 洋 ，孙 梦 （1.西华大学生物工程学院，四川 成都 610039 ；2.成都大帝汉克生物科技有限公司，四川 成都 611130 ） 摘  要：为将液体香精加工成缓释性固体粉末香精，以明胶和海藻酸钠为壁材，用复凝聚法制备液体香精微胶囊， 以包埋率为评价指标，研究了不同因素对微胶囊制备效果的影响，通过正交试验优化工艺条件。结果表明，壁材为 质量分数1% 明胶溶液与0.5%海藻酸钠溶液按3 ∶1的质量比混合、液体香精与壁材质量比1∶1、复凝pH 4.0～4.5条件 下，平均包埋率达92.80%，对包埋率影响最大的因素为两壁材的比例。用石油醚浸提，与同类固体粉末香精对比考 察，验证了所包埋的微胶囊具有较好的缓释效果。 关键词：液体香精；微胶囊；复凝聚法；明胶；海藻酸钠 Application of Orthogonal Array Design to Optimize the Preparation of Liquid Essence Microcapsules by Complex Coacervation 1 1 2 1 1 BAO Qingbin , WANG Chunli , KONG Ling , HE Yang , SUN Meng (1. School of Bioengineering, Xihua University, Chengdu 610039, China; 2. DadHank (Chengdu) Biotech Corp., Chengdu 611130, China) Abstract: The preparation of sustained-release essence powder by microencapsulating liquid essence with gelatin and sodium alginate as the coating materials using a complex coacervation method was optimized for improved embedding rate using an orthogonal array design. It was demonstrated that the average embedding rate was 92.80% when the complex coacervation was carried out at pH ranging from 4.0 to 4.5 using a coating material consisting of a 3:1 (by mass) mixture of 1% gelatin and 0.5% sodium alginate with a ratio of wall material to core material of 1:1 (by mass). The ratio between the two coating materials was found to be the factor with the greatest influence on embedding efficiency. Compared with ordinary essence powder after extraction with petroleum ether, the m