鸡卵巢组织基因差异表达与繁殖性状杂种优势的关系研究毕业论文-动物遗传育种与繁殖专业论文.docxVIP

中凰农业大学蚺士学位论文 中凰农业大学蚺士学位论文 ABSTRACT I Abstract In order to understand the molecular basis ofchicken heterosis in reproduction trails,mRNA differential display melhod and real time fluorescence quantify reverse transcriptional polymerase chain reaction (FQRT-PCR)were used to analyze the differential gene expression of ovary tissue between hybrids and their parental tines iu a 4x4 diallel CROSS，involving 4 chicken breeds，which were White Plymouth Rock(E)CAU Brown(D)，Silkies(C)and White Leghorn(A)． (1)On the basis ofDDRT-PCR technique we found that： Total of 331 differential displayed eDNA bands from 1161 Were displayed in the 4×4 diallel cross combinations with 30 pairs ofprimers，which shows the differences ofgene expression between hybrids and their parental lines were very obvious in quantity and quality．Seven types ofdifferential expression patterns were found：Co‘dominance expressed pattem(T1)，Underexpression of parental fragemenIs in hybrids(T2)，Over-expression ofparental fragements in hybrids汀3)’Hybrid—absence expressed pattem (T4)，Single parent—specific expressed pattern fr5)，Dominant expression ffagements of single parent in hybrids(T6)，Hybrid-specific expressed pattern(T7)． Correlation analysis indicated that there were significant correlation between the pattern ofT3 and the heternsis percentage of egg number of 32·week and 42*week old po．01)，while there arc negative significant correlation between the pattem ofT7 and the heterosis percentage ofegg number of 32-week and 42 week-old 00 01)． Of 18 differential expressed sequences conirmed by relwerse Northern dot blot sequenced，OBe WBS in high homology with encoded mRNA sequences with known functions(e=0)；Other 17 sequences， without homologous sequences by BLAST analysis，were new EST sequences，The sequence of differentially expressed CtPA—like had high similarity with human tissue type plasminogen activator (tPA)(