里氏拟石磺线粒体基因组与石磺科四属种贝类转录组分析.pdfVIP

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里氏拟石磺线粒体基因组与石磺科四属种贝类转录组分析.pdf

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上海海洋大学硕士论文目录摘要······························································································ Ⅰ ABSTRACT ·······················································································Ⅲ 引言································································································1 石磺科贝类简介··············································································1 线粒体全基因组分析········································································1 转录组测序 ····················································································2 表达谱 ··························································································2 第一章里氏拟石磺线粒体全基因组分析····················································4 1 材料与方法················································································4 1.1 实验材料···········································································4 1.2 实验方法···········································································6 2 结果·························································································6 3 讨论·························································································8 第二章石磺科四属种贝类的实验材料与转录组分析方法·······························9 1 实验材料···················································································9 2 实验流程·················································································10 3 数据分析·················································································10 第三章石磺科四属种贝类转录组分析···················································13 1 De novo RNA 转录组组装拼接与结果统计 ···································13 1.1 转录组样本测序数据产出量统计··················································13 1.2 转录组测序数据的组装拼接·····························