猫上丘年龄相关性形态学变化研究-细胞生物学专业毕业论文.docxVIP

猫上丘年龄相关性形态学变化研究 摘 要 上丘(Superior Colliculus , SC)是哺乳动物中脑顶部的一个分层结构， 它不仅是视觉通路的重要反射中枢，而且还参与听觉、躯体感觉等多种 生理活动。其老年性结构及功能变化可能是上丘水平视觉功能衰退的重 要因素之一。本文比较研究了青年猫和老年猫上丘年龄相关的形态学变 化。 1. 结合 Nissl 法和 S100 免疫组织化学方法(SABC 法)观察和比较了 猫上丘各灰质层中神经元及 S100 在上丘中的分布及其表达的年龄相关 性变化。结果显示，S100 蛋白在猫上丘中广泛分布,灰质层中表达强于 白质层; 与青年猫相比，老年猫上丘白质层无显著变化, 灰质层中 S100 蛋白表达显著增强(P0.01)，胞体较大，阳性较强;灰质层(浅灰质层、中 灰质层、深灰质层) 神经元密度显著下降(P0.01)。提示在动物衰老过程 中，上丘灰质层存在着明显的星形胶质细胞反应性增生，这种增生可能 对维持老年个体上丘形态结构和延缓老年动物上丘功能衰退具有积极 意义。 2. 采用免疫组织化学方法，对青年猫和老年猫两年龄组个体的上 丘表浅层神经元及 GABA 能神经元进行形态学观察和定量比较，结果表 明 GABA 阳性反应神经元、阳性纤维及其终末在青年猫及老年猫上丘表 浅层均有分布。与青年猫相比，老年猫上丘表浅层 GABA 能神经元密度 均显著下降(P0.01)；GABA 能神经元胞体直径明显减小(P0.01)，阳性 反应明显减弱。提示在动物个体衰老进程中，老年个体上丘 GABA 能神 经元对视觉信息传递及整合过程的抑制性调节功能削弱，可能是上丘水 平上导致老年个体视觉功能衰退的原因之一。 关键词：猫；上丘；上丘表浅层；视觉功能衰退；GABA；S100 蛋白 Studies on Age-Related Morphological Changes in the Superior Colliculus of Cats Abstract The Superior Colliculus (SC) is a lamina structrue of the mammalian midbrain. It is not only the reflect center of the visul circuit,but also involved in auditory, body feeling and so on. The changes of its senile structure and function at the SC level are possibly one of the important factors which lead to the decline of visual function. This thesis makes a comparative study of the age differences between the young cats and the old cats at the level of SC. Nissl stain was used to exhibit the structure and neurons, and immunohistochemical method (SABC method) was applied to exhibit the S100-IR cells. Results showed that S100 protin was wide distributed in the cat SC, and S100-IR cells in the gray layers were denser than in the white layers. Compared with that of young cats, neurons density in gray layers in old ones decreased while the S100-IR cells in old ones increased in the density, swelled in the body, enhanced in the immunoreactivity. The SC astrocytes in gray layers in old animals have obvious reactive increasing, which may have positive influences to maintain the morphological structure of SC and detain the visual degenerat