鱼皮中胶原蛋白的提取论文.doc

鱼皮中胶原蛋白的提取 摘要 胶原蛋白是构成动物支持组织的结构蛋白质,其提取制品已广泛应用于医药、保健、食品加工、化妆品等众多领域,迄今其制品主要是从一些陆生哺乳动物如牛、鱼等的皮肤提取。但近年来，科学家对鱼皮中胶原蛋白的提取也没有止步，“鱼皮”含有丰富的蛋白质和多种微量元素，其蛋白质主要是大分子的胶原蛋白及粘多糖的成分，是女士养颜护肤美容保健佳品，也是滋补佳品。提取方法主要有聚能式脉冲逆流超声辅助法、中性盐萃取法、酶法、酸法、碱法，而这些方法都尚未获得较高的提取率，但其中酶法提取对胶原蛋白破坏性小，能更好、更完全地得到活性胶原蛋白，具有反应速度快、时间短、无环境污染等优点。超声波提取因其空穴效应、污染小、节能、环保等优越性能在当今的生产、生活和科学研究中被广泛使用。所以本论文以新鲜鲫鱼皮为原料，以胶原蛋白提取率为目标参数，采用超声波提取法对鱼皮中胶原蛋白提取，从而进一步提高鱼皮的药用、食用价值。选取提取时间、料液比、提取功率3个影响因子进行单因素实验。在单因素实验设计的基础上利用正交试验，得鱼皮中胶原蛋白提取的最佳工艺条件。结果表明，当提取时间为55min、料液比为1:45、提取功率为100%时候提取率最高为 关键字：鲫鱼皮；超声波提取法；胶原蛋白；正交试验 Optimization Of Extraction of collagen from skin Abstract : Protein collagen is animal support organization structure, its products have been widely used in medicine, health care, food processing, cosmetics and many other fields, so far its products mainly from terrestrial mammals such as cows, pigs and other skin extract. But in recent years, scientists on the extraction of collagen from skin did not stop, skin is rich in proteins and other trace elements, proteins are large molecules of collagen and glycosaminoglycan constituents, it is beauty skin care and beauty-care to share, and tonic. Extraction methods have shaped Pulse counter ultrasound-assisted, neutral salt extraction, enzymatic method, acid, alkali, all of which have not been high extraction rate, but less damaging enzymatic extraction of collagen protein, can provide better, more fully active collagen, high response speed, time is short, no environmental pollution and so on. Ultrasonic wave extraction from the hole effect, pollution, energy saving, environmental protection and other advantages in todays production and daily life and is widely used in scientific research. Fresh fish as raw material this thesis, aimed at collagen extraction rate parameters, extracted by ultrasonic wave extraction of collagen in the skin, so as to further enhance the value of medicinal, edible skin. Select time, solid-liquid ratio, extraction power 3 factors of single factors. Singl