慢性间断性缺氧对子代雄性大鼠血清血管紧张素Ⅱ及脂联素水平的影响-内科学(心血管)专业毕业论文.docxVIP

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2019-05-18 发布于上海
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慢性间断性缺氧对子代雄性大鼠血清血管紧张素Ⅱ及脂联素水平的影响-内科学(心血管)专业毕业论文.docx

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- - PAGE 10 - Effects of chronic intermittent hypoxia on angiotensin II and adiponectin in offspring of male rats Abstract Objective To explore the effect of chronic hypoxia on serum adiponectin and angiotensin II by the model of chronic intermittent hypooxia in rats. Methods SD pregnant rats were in closed box under monitoring of oxygen concentration measurement instrument. Blowing time and flow of all kinds of gases were preset, so as to control oxygen concentration in closed box. We set 4 minutes per cycle (hypoxia /reoxygenation ) , gases blowed into the box and time set were as follows: nitrogen 90 seconds-resting 30 seconds-oxygen 30 seconds-air90 seconds. With the minimum oxygen concentration （10±0.5）% in closed box and the maximum oxygen concentration （20±1.0）%,oxygen concentration in one cycle was recorded every 5 seconds. 5 cycles was continuously recorded, which was maintained for 8 hours. Carbon dioxide and water vapor was absorbed by soda lime and calcium chloride, respectively, while feed and water was as usual. Then the listed procedure was repeated until pregnancy. The offspring of the pregnant rats was handled by hypoxia again, with 8 hours oxygen deficit per day for 12 successive weeks. AngII was measured by ELISA, and the data was compared by two-way ANOVA test. Results We found that the levels of AngII in offspring from intrauterine hypoxia was much higher than that in offspring from pregnant rats with normal oxygen (2.39±0.45 pg/mL vs. 2.06±0.12 pg/mL, p0.05), but the level of ADP was lower（12.03±1.14 mg/L vs. 14.15±1.72 mg/L, p0.05）.For offspring with hypoxia, the level of AngII in rats from mothers with intrauterine hypoxia was much lower than that in rats from mothers with normal oxygen（2.07±0.17 pg/mL vs. 2.50±0.34 pg/mL, p0.05）, but the levels of ADP was much higher that latter （14.60±0.32 mg/L vs. 11.51±1.98 mg/L, p0.05）. Conclusions Intrauterine hypoxia could change the levels of hormone in offspring, inducing the increase of AngII and d