锯缘青蟹精氨酸激酶的分离纯化、分子克隆及过敏原性研究-微生物学专业论文.docxVIP

II II 匹配分值最高为 80，吻合肽段数为为 12 条，确认纯化的天然蛋白及融合表达的重组蛋白 均为精氨酸激酶。 应用甲壳类过敏患者血清的免疫印迹分析，结果显示纯化的天然蛋白及融合表达的重 组蛋白具有免疫原性，首次证实精氨酸激酶为锯缘青蟹的特异性过敏原。应用体外重组表 达融合蛋白的胶体金免疫试纸条分析，结果表明该融合蛋白可用于甲壳类食物过敏诊断试 剂的开发与应用。 关键词 锯缘青蟹，精氨酸激酶，过敏原，纯化，克隆表达，双向电泳，质谱分析，免疫 胶体金层析 III III Arginine Kinase from Sc ylla se rrata : Purification, Cloning, Expression and Allergenic Analysis Abstract Food allergen is a common immunity disease. With the development of society and globalization, the rate of food allergen was higher and higher, and became a global attention food safety problem. Food allergen was mainly caused by proteins in food, the s