枇杷属野生植物种胚离体培养和植株再生研究-亚热带植物科学.pdfVIP

2016,45(2): 112 ～116. Subtropical Plant Science 枇杷属野生植物种胚离体培养和 植株再生研究 刘义存 1,2 2 2 ，黄天启 ，林顺权 (1.仲恺农业工程学院园艺园林学院，广东 广州 510225 ；2.华南农业大学园艺学院，广东 广州 510642) 摘 要：以枇杷属Eriobotrya 植物 11 个野生种、1 个远缘杂交后代材料为试材，采用胚为外植体，在不同植物 生长调节剂配比培养条件下，进行胚离体培养和植株再生研究。结果表明，胚培养的最佳培养基配方为 MS + 6-BA 1.0 mg·L-1 + KT 1.0 mg·L-1 + NAA 0.1 mg·L-1 。在此培养基中，所有试验材料均获得成功，其中栎叶枇杷 E. prinoides 的萌发率和丛芽率均达 100%，有效地建立了枇杷属野生种的胚离体培养及其植株再生体系。 关键词：枇杷属植物；野生种；胚培养；植株再生 Doi: 10.3969/j.issn.1009-7791.2016.02.003 中图分类号：S667.3 文献标识码：A 文章编号：1009-7791(2016)02-0112-05 Studies on in vitro Culture and Regeneration of Embryos in Wild Plants of Eriobotrya 1,2 2 2 LIU Yi-cun , HUANG Tian-qi , LIN Shun-quan (1.College of Horticulture and Landscape Architecture, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, Guangdong China; 2.College of Horticulture, South China Agricultural University, Guangzhou 510642, Guangdong China) Abstract: The effects of the combination of the plant growth regulators and culture condition on the in vitro culture and regeneration of the embryo of Eriobotrya plants were studied. The results showed -1 -1 -1 that the best media in vitro was MS + 6-BA 1.0 mg·L + KT 1.0 mg·L + NAA 0.1 mg·L for embryo culture of 11 wild species and 1 intergeneric hybrid of Eriobotrya plants. All of the explants were succeed in regeneration from embryo culture, among them, the germination rate and sprouting