应用高效液相色谱.doc

· PAGE 1414· 药学学报 Acta Pharmaceutica Sinica 2010, 45 (11): 1410?1414 李蓓佳等: 应用高效液相色谱-质谱联用技术研究通脉颗粒的指纹图谱 · PAGE 1413· · PAGE 1410· 药学学报 Acta Pharmaceutica Sinica 2010, 45 (11): 1410?1414 应用高效液相色谱-质谱联用技术研究通脉颗粒的指纹图谱 李蓓佳1, 2, 向 诚1, 杨秀伟1, 吴立军2, 果德安1, 叶 敏1* (1. 北京大学药学院天然药物与仿生药物国家重点实验室, 北京 100191; 2. 沈阳药科大学中药学院, 辽宁 沈阳 110016) 摘要: 为了比较全面并且快速地阐明中药复方通脉颗粒的化学组成, 本文建立了通脉颗粒的高效液相色谱 (HPLC-UV) 指纹图谱, 并利用液相-二极管阵列-质谱联用 (LC-DAD-MS) 技术, 从通脉颗粒中鉴定了22个化合物, 并归属了各化合物的单味药来源。结果显示通脉颗粒的主要成分包括染料木素8-C-葡萄糖苷、葛根素、大豆素8-C-芹糖 (1, 6)-O-葡萄糖苷、大豆苷以及丹酚酸B。又对10批来自不同生产厂家的样品进行HPLC分析, 发现各样品的化学组成相似, 但主要色谱峰的面积差异显著。该研究比较全面地阐明了通脉颗粒的化学组成, 为此中药复方制剂的质量控制奠定基础。 关键词: 通脉颗粒; 指纹图谱; HPLC-DAD-MS 中图分类号: R917 文献标识码: A 文章编号: 0513-4870 (2010) 11-1410-05 Fingerprints of Tongmai Keli by HPLC-DAD-MS LI Bei-jia1, 2, XIANG Cheng1, YANG Xiu-wei1, WU Li-jun2, GUO De-an1, YE Min1* (1. The State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China; 2. College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, China) Abstract: In order to clarify the chemical composition of Tongmai Keli, a HPLC fingerprint was established, and the 22 peaks were characterized by LC-DAD-MS. The herbal sources of these peaks were assigned. The results implied that genistin 8-C-glucoside, puerarin, daidzein 8-C-apiosyl (1, 6)-O-glucoside, daidzin, and salvianolic acid B were the main constituents of in Tongmai Keli. Ten batches of Tongmai Keli produced by different pharmaceutical companies were analyzed. Although different batches contained similar compounds, the contents of major compounds were significantly different. The method established in this study could be used for the quality control of Tongmai Keli. Key words: Tongmai Keli; fingerprint; HPLC-DAD-MS 收稿日期: 2010-03-20.基金项目: 国家“重大新药创制”科技重大专项资助项目 (2009ZX09502-006