碱性蛋白酶酶解罗非鱼下脚料蛋白动力学研究-食品科学.pdf

※工艺技术 食品科学 2012, Vol. 33, No. 02 53 黄秋霞，孙建华，兰雄雕，蒋林斌，童张法，廖丹葵 * (广西大学化学化工学院，广西石化资源加工及过程强化技术实验室，广西 南宁 530004) 　　 以加工罗非鱼片废弃的下脚料蛋白和碱性蛋白酶为原料，以水解度为指标，考察温度、底物质量浓度、 酶用量及pH 等因素对碱性蛋白酶酶解罗非鱼下脚料蛋白水解度的影响。得到碱性蛋白酶水解罗非鱼下脚料蛋白的 适宜工艺条件为：酶解温度 50 ℃、底物质量浓度20g/L 、酶用量为0.067g/L 、pH9.50 、反应时间 180min，此时 水解度为 22.00% 。同时得到50 ℃条件下酶失活常数Kd 为 37.9min-1 ，反应速率常数K2 为244.2min-1 、米氏常数KM 为29.27g/L 、最大反应速度 Vmax 为28.41g/(min ·L) ；并建立了相应的水解动力学模型。验证实验表明：建立的水 解动力学模型与实际水解过程基本吻合。 罗非鱼下脚料；碱性蛋白酶；酶解；动力学 　　 Kinetic Model for Enzymatic Hydrolysis of Protein from Tilapia Waste 　　 HUANG Qiu-xia ，SUN Jian-hua，LAN Xiong-diao ，JIANG Lin-bin ，TONG Zhang-fa ，LIAO Dan-kui* (School of Chemistry and Chemical Engineering, Guangxi University, Guangxi Petrochenical Resources Processing and Process Intensification Technology, Nanning 530004, China) 　　 Abstract：Tilapia waste was used as the material to conduct hydrolysis by alkaline protease. The degree of hydrolysis (DH) of protein from tilapia waste was determined to explore the optimal enzymatic hydrolysis conditions. The results indicated that the optimal hydrolysis conditions for tilapia waste were hydrolysis temperature of 50 ℃, substrate concentration of 20 g/L, enzyme concentration of 0.067 g/L, hydrolysis pH of 9.50 and hydrolysis time of 180 min. Under the optimal hydrolysis conditions, the DH of tilapia waste was 22%. Meanwhile, a kinetic model of hydrolysis was established, which revealed an -1 -1 inactivation constant (Kd) of 37.9 min for enzyme, enzymatic reaction rate constant (K2) of 244.2 min , Michaelis-Menten constant (KM) of 29.27 g/L, and the maximum reaction rate ( Vmax) of 28.41 g/