单细胞基因组和转录组的共测定方法研究进展-中国现代应用药学.PDF

·综 述 · 单细胞基因组和转录组的共测定方法研究进展  万睿璇 1 ，周抒遥 1 ，徐欢笛 1 ，汪浩宇 1 ，娄云阁 1 ，姜净 1 ，邹秉杰 2 ，宋沁馨 1,2* ，周国华 1,2(1. 中国药科大 学药物质量与安全预警教育部重点实验室，南京 210009 ；2.南京军区南京总医院药理科，南京 210002) 摘要 ：单细胞测序提供了大量样品测序所不能提供的异质性等信息。然而由于细胞生长发育受多种因素调节，仅依靠基 因型或者表型信息无法全面、准确地说明问题。因此，开发单细胞基因组转录组共测定技术为研究基因变异与表型变异 间的关系提供了强有力的工具。目前，单细胞基因组与转录组共测定技术主要分为全细胞裂解法(如：GT-Seq、DR-Seq) ， 细胞膜裂解法(如：SIDR、微流控芯片法) 以及其他方法(如：Simul-Seq、Gel-Seq) 。本文对这几类技术进行了简要介绍， 并对其前景进行了展望。 关键词：单细胞测序；转录组；基因组；单细胞多组学分析 中图分类号：R963 文献标志码：A 文章编号：1007-7693(2018)09-1423-08 DOI: 10.13748/j.cnki.issn1007-7693.2018.09.035 引用本文：万睿璇, 周抒遥, 徐欢笛, 等. 单细胞基因组和转录组的共测定方法研究进展[J]. 中国现代应用药学, 2018, 35(9): 1423-1430. Progress in Parallel Sequencing of the Genomes and Transcriptomes of Single Cells 1 1 1 1 1 1 2 WAN Ruixuan , ZHOU Shuyao , XU Huandi , WANG Haoyu , LOU Yunge , JIANG Jing , ZOU Bingjie , SONG Qinxin1,2*, ZHOU Guohua1,2(1.Key Laboratory of Drug Quality Control and Pharmacovigilance of Ministry of Education, China Pharmaceutical University, Nanjing 210009, China; 2.Department of Pharmacology, Nanjing General Hospital, Nanjing 210002, China) ABSTRACT: Single-cell sequencing provides information, such as heterogeneity, that is omitted by bulk sample sequencing. However, since cell growth and development are regulated by many factors, relying on genotype or phenotype information alone is not able to comprehensively and accurately explain the problem. Co-detection of single-cell DNA-Seq and RNA-Seq offers a strong tool for researchers on the aspect of the relationship between gene variations and phenotype variations. Up to present, there are strategies described below have been employed: whole cell lysis (e.g.: GT-Seq, DR-Seq), plasma membr