青岛潮间带沉积物厌氧细菌的分离培养和多样性研究.pdfVIP

Study on Isolation and Diversity of Culturable Anaerobic Bacteria in Intertidal Sediment of Qingdao Abstract Anaerobic bacteria are a large group of marine microorganism that lives better without oxygen. Obligate anaerobic bacteria is liable to be damaged by oxygen, even to dead due to lacking superoxide dismutase (SOD), which makes them more harder to be cultured. However, as a large group of marine microorganism, anaerobic bacteria play an important role in biogeochemical cycles, bioremediation, exploitation of green energy, regulation of global climate and isolation of new drugs, etc. Intertidal falt is the largest transition zone between continent and sea and abounds in biological resource, which is severely affected by various physical and chemical factors as well as activities of human. It is characterized by a high nutrient input from the land and open sea. This nutrient supplyment stimulates intense benthic primary production, fuelling microbial activities in the upper sediment layers, which makes the lower layer an anaerobic expanse with abundant anaerobic bacteria. However, by now, researchers mainly focused on aerobic cultivation, and little informations about culturable anaerobic bacteria inhabiting in the areas is available. Anaerobic bacteria of the lower layer play a key role in biogeochemical cycles with more research values. To test the easy method we designed and several media (2216E medium and 4 kinds of SPG media) working for various anaerobic bacteria for further studying the function of anaerobes in the biogeochemical cycle, diversity of cultura