林可霉素菌渣堆肥抗生素抗性基因变化分析-中国环境科学.pdf

中国环境科学 2018,38(11) ：4276~4283 China Environmental Science 林可霉素菌渣堆肥抗生素抗性基因变化分析 任省涛 1,郭夏丽 1,芦阿虔 1,张倩倩 1,郭笑盈 1,王 岩 1*,王连忠 2,张宝宝 2 (1.郑州大学化工与能源学院,河南 郑州 450001 ；2.河南省新乡华星制药厂,河南 新乡 453731) 摘要：为了研究抗生素菌渣堆肥过程中抗生素抗性基因(ARGs) 的变化情况, 以林可霉素菌渣-糠醛渣堆肥为研究对 , 以污泥-糠醛渣堆肥为对照.运用 荧光定量 PCR 技术检测到了堆肥过程中 lnuA-01、sul 1、ermA 、ermB 、ermC 等 5 种林可霉素抗性基因和整合子基因 intI1 的变化情况.结果表明,堆肥 化处理可以降解 99%的林可霉素残留,两者堆肥 ARGs 总量绝对丰度均有较大增加,而相对丰度降低 5%~22%.同时发现林可霉素菌渣堆肥有助于 intI1 的富集,表明林可霉素菌渣堆肥存在生态风险.冗余分析显示, ARGs 变化受环境因子影响严重,影响顺序为 pH 值林可霉素残留温度C/N. 关键词：堆肥；抗生素；林可霉素菌渣；荧光定量PCR；ARGs 中图分类号：X172 文献标识码：A 文章编号：1000-6923(2018)11-4276-08 1 1 Variations of Antibiotic resistance genes during lincomycin mycelia dreg composting. REN Sheng-tao , GUO Xia-li , LU A-qian1 1 1 1* 2 2 , ZHANG Qian-qian , GUO Xiao-ying , WANG Yan , WANG Lian-zhong , ZHANG Bao-bao (1.College of Chemical Engineering and Energy, Zhengzhou University, Zhengzhou 450001, China ；2.Henan Xinxiang Hua Xing Pharmaceutical Factory, Xinxiang 453731, China). China Environmental Science, 2018,38(11)：4276~4283 Abstract ：In order to investigate the variation of antibiotic resistance genes during the antibiotic mycelia dregs composting process, five antibiotic resistance genes (lnuA-01, sul 1, ermA, ermB, ermC) and one horizontal gene transfer (intI1) were detected from the compost by quantitative PCR technique. The results showed that 99% lincomycin was degraded after composting. For both the lincomycin mycelia dreg compost and sewage sludge compost, the absolute total of ARGs was all increased. By contrast, the relative total of ARGs were all decreased b