海洋产电菌ShewanellamarisflaviEP1的脱色特性-Core.pdfVIP

微生物学通报 JAN 20, 2011, 38(1): 2−7 Microbiology China © 2011 by Institute of Microbiology, CAS tongbao@ 研究报告 海洋产电菌 Shewanella marisflavi EP1 的脱色特性 1 2* 3 邹聪慧 徐方成 陈新华 (1. 361005) (2. 361005) (3. 361005) 摘 要: 以一株新筛选得到的海洋产电菌Shewanella marisflavi EP1 作为实验材料, 研究了该菌株 关于偶氮、蒽醌、三苯基甲烷等染料的脱色能力及脱色机制。结果表明, 该菌株对这些染料均具 有较好的脱色能力, 最高脱色容量达到925 mg 染料/(g 细胞干重·d)。EP1 能利用葡萄糖、蔗糖、 木糖、乳酸、甲酸、柠檬酸等多种碳源将单偶氮染料丽春红 2R 脱色。脱色的 pH 、温度和NaCl 浓度范围分别是: pH 6−10、15 °C−40 °C 、0−8%。最优脱色条件: 乳酸, pH 8、35 °C 、1%−2% NaCl, 10 h 内脱色率高达99.95% 。分光光谱结果表明, 在 0-8% NaCl 浓度范围内EP1 脱色机制为降解 脱色。 关键词: 产电菌, Shewanella marisflavi EP1, 偶氮染料, 生物脱色 Decolorization of dyes by a current-producing bacterium Shewanella marisflavi EP1 isolated from sea sediments ZOU Cong-Hui1 XU Fang-Cheng2* CHEN Xin-Hua3 (1. College of Oceanography and Environmental Science, Xiamen University, Xiamen, Fujian 361005, China) (2. College of Chemistry and Chemical Engineering, Xiamen University, Xiamen, Fujian 361005, China) (3. Key Laboratory of Marine Biogenetic Resources , Third Institute of Oceanography, State Oceanic Administration , Xiamen, Fujian 361005, China) Abstract: In this study, we investigated a new current-producing strain, Shewanella marisflavi EP1 iso- lated from costal sediments of Xiamen, its decolorization capability and mechanism of several types of dyes such as azo, anthraquinone and triphenyl methane. The results indicated that these dyes cou